2019 journal article

In vitro assessment of the long-term stability of the inhibitory effects of canine and feline plasma on MMP 2 and 9

VETERINARY OPHTHALMOLOGY, 22(6), 898–901.

By: M. Lively n & H. Westermeyer n

co-author countries: United States of America 🇺🇸
author keywords: corneal malacia; matrix metalloproteinase inhibition; MMP 2; MMP 9; plasma stability; ulcerative keratitis
MeSH headings : Animals; Cats / blood; Dogs / blood; Matrix Metalloproteinase 2 / blood; Matrix Metalloproteinase 9 / blood; Matrix Metalloproteinase Inhibitors; Plasma / enzymology; Specimen Handling; Time Factors
Source: Web Of Science
Added: December 16, 2019

Abstract Purpose To evaluate the effect of increasing storage time on the inhibitory effects of canine and feline plasma on matrix metalloproteinases (MMP) 2 and 9 in vitro. Methods Matrix metalloproteinases 2 and 9 activity in the presence of canine plasma stored for 57, 155, 222, 316, 367, and 438 days, and feline plasma stored for 17, 198, 565, and 954 days was assayed using a commercially available colorimetric assay kit. Results For canine plasma, the MMP 2 activity for older samples was not significantly different than the 57‐day sample ( P = 0.2025‐0.9033). Two canine samples had significantly lower MMP 9 activity than the 57‐day sample (367 days: P = 0.0099 , 438 days: P = 0.0348, others P = 0.0778‐0.9928). For feline plasma, storage time did not significantly affect inhibition of MMP 2 and MMP 9 activity (ANOVA, P = 0.2688 and P = 0.2404, respectively). Conclusions Increasing storage time does not significantly decrease the inhibiting activity of plasma on MMP 2 and 9 for up to 14 months in dogs and 31 months in cats.