2020 review

Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor

[Review of ]. CELL JOURNAL, 22(2), 133–139.

By: S. Bahrami*, A. Amiri-Yekta*, A. Daneshipour*, S. Jazayeri*, P. Mozdziak n, M. Sanati*, H. Gourabi*

co-author countries: Iran (Islamic Republic of) 🇮🇷 United States of America 🇺🇸
author keywords: Chickens; CRISPR/Cas9; Ovalbumin; Recombinant Protein; Transgenes
Source: Web Of Science
Added: December 16, 2019

Specific developmental characteristics of the chicken make it an attractive model for the generation of transgenic organisms. Chicken possess a strong potential for recombinant protein production and can be used as a powerful bioreactor to produce pharmaceutical and nutritional proteins. Several transgenic chickens have been generated during the last two decades via viral and non-viral transfection. Culturing chicken primordial germ cells (PGCs) and their ability for germline transmission ushered in a new stage in this regard. With the advent of CRISPR/Cas9 system, a new phase of studies for manipulating genomes has begun. It is feasible to integrate a desired gene in a predetermined position of the genome using CRISPR/Cas9 system. In this review, we discuss the new approaches and technologies that can be applied to generate a transgenic chicken with regards to recombinant protein productions.