2010 journal article

Oil content and fatty acid composition variability in wild peanut species

PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION, 8(3), 232–234.

By: M. Wang*, N. Barkley*, M. Chinnan, H. Stalker n & R. Pittman*

author keywords: fatty acid composition; oil content; peanut germplasm; real-time PCR genotyping; wild species
TL;DR: Oil content was measured using nuclear magnetic resonance, fatty acid composition was analysed using gas chromatography, and the D150N functional mutation of the FAD2A gene was screened by real-time PCR, and results were consistent. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

Wild peanut species are useful genetic resources for improving the levels of disease/pest resistance and for enhancing the quality of seed composition by interspecific hybridization. The variation in oil content and fatty acid composition of wild peanut species in the United States Department of Agriculture germplasm collection is unknown. Seeds available from 39 wild species (plus a cultivated peanut) were requested from the U.S. peanut germplasm collection. Oil content was measured using nuclear magnetic resonance, fatty acid composition was analysed using gas chromatography, and the D150N functional mutation of theFAD2Agene was screened by real-time PCR. Significant variability in oil content (41.7–61.3%) was identified among the wild peanut species.Arachis magnacontained significantly more oil (61%) than cultivated peanut (56%). There was no functional mutation identified within theFAD2Agene target, and no wild species were identified with a high ratio of oleic acid to linoleic acid. The results from gas chromatography and real-time PCR analyses were consistent. However,Arachis sylvestriscontained a significantly higher amount (22%) of long-chain fatty acid (LCFA) than the cultivated peanut (4%). Thus,A.magnaandA. sylvestrismay be good breeding materials to use for increasing oil content or LCFA composition of cultivated peanuts in breeding programs.