2014 journal article

Disruption of microalgal cells using high-frequency focused ultrasound

BIORESOURCE TECHNOLOGY, 153, 315–321.

By: M. Wang n, W. Yuan n , X. Jiang n, Y. Jing n & Z. Wang n

co-author countries: United States of America πŸ‡ΊπŸ‡Έ
author keywords: Microalgae; Ultrasound; Cell disruption; Lipid extraction
MeSH headings : Cell Fractionation / methods; Chlorophyll / metabolism; Chlorophyll A; Colony Count, Microbial; Fluorescence; Lipids / chemistry; Microalgae / cytology; Microalgae / growth & development; Microalgae / metabolism; Oxazines / metabolism; Staining and Labeling; Ultrasonics / instrumentation; Ultrasonics / methods
Source: Web Of Science
Added: August 6, 2018

The objective of this study was to evaluate the effectiveness of high-frequency focused ultrasound (HFFU) in microalgal cell disruption. Two microalgal species including Scenedesmus dimorphus and Nannochloropsis oculata were treated by a 3.2-MHz, 40-W focused ultrasound and a 100-W, low-frequency (20kHz) non-focused ultrasound (LFNFU). The results demonstrated that HFFU was effective in the disruption of microalgal cells, indicated by significantly increased lipid fluorescence density, the decrease of cell sizes, and the increase of chlorophyll a fluorescence density after treatments. Compared with LFNFU, HFFU treatment was more energy efficient. The combination of high and low frequency treatments was found to be even more effective than single frequency treatment at the same processing time, indicating that frequency played a critical role in cell disruption. In both HFFU and LFNFU treatments, the effectiveness of cell disruption was found to be dependent on the cell treated.