2022 journal article

Ca2+ signaling-mediated low-intensity pulsed ultrasound-induced proliferation and activation of motor neuron cells

ULTRASONICS, 124.

By: T. Truong*, W. Chiu*, Y. Lai*, H. Huang*, X. Jiang n & C. Huang n

co-author countries: Taiwan, Province of China 🇹🇼 United States of America 🇺🇸
author keywords: Low-intensity pulsed ultrasound; Motor neuron disease; NSC-34 cell line; Neurite outgrowth; Morphology; Ca (2+) signaling; Ca (2+) -dependent transcription factors
MeSH headings : Animals; Calcium Signaling; Cell Proliferation; Mice; Motor Neurons / physiology; NF-kappa B; Ultrasonic Waves
Source: Web Of Science
Added: May 16, 2022

Motor neuron diseases (MND) including amyotrophic lateral sclerosis and Parkinson disease are commonly neurodegenerative, causing a gradual loss of nerve cells and affecting the mechanisms underlying changes in calcium (Ca2+)-regulated dendritic growth. In this study, the NSC-34 cell line, a population of hybridomas generated using mouse spinal cord cells with neuroblastoma, was used to investigate the effect of low-intensity pulsed ultrasound (LIPUS) as part of an MND treatment model. After NSC-34 cells were seeded for 24 h, LIPUS stimulation was performed on the cells at days 1 and 3 using a non-focused transducer at 1.15 MHz for 8 min. NSC-34 cell proliferation and morphological changes were observed at various LIPUS intensities and different combinations of Ca2+ channel blockers. The nuclear translocation of Ca2+-dependent transcription factors was also examined. We observed that the neurite outgrowth and cell number of NSC-34 significantly increased with LIPUS stimulation at days 2 and 4, which may be associated with the treatment's positive effect on the activation of Ca2+-dependent transcription factors, such as nuclear factor of activated T cells and nuclear factor-kappa B. Our findings suggest that the LIPUS-induced Ca2+ signaling and transcription factor activation facilitate the morphological maturation and proliferation of NSC-34 cells, presenting a promising noninvasive method to improve stimulation therapy for MNDs in the future.