2017 journal article

A VIGS screen identifies immunity in the Arabidopsis Pla-1 accession to viruses in two different genera of the Geminiviridae

PLANT JOURNAL, 92(5), 796–807.

By: M. Reyes n, M. Flores-Vergara n, O. Guerra-Peraza n, C. Rajabu*, J. Desai n, Y. Hiromoto-Ruiz n, J. Ndunguru*, L. Hanley-Bowdoin n ...

co-author countries: Tanzania, United Republic of 🇹🇿 United States of America 🇺🇸
author keywords: Arabidopsis; BCTV; CaLCuV; geminivirus; gip-1; immunity; Pla-1; VIGS
MeSH headings : Arabidopsis / virology; Geminiviridae / immunology; Gene Silencing; Plant Diseases / immunology; Plant Diseases / virology; Plant Immunity; Quantitative Trait Loci / genetics
Source: Web Of Science
Added: August 6, 2018

Summary Geminiviruses are DNA viruses that cause severe crop losses in different parts of the world, and there is a need for genetic sources of resistance to help combat them. Arabidopsis has been used as a source for virus‐resistant genes that derive from alterations in essential host factors. We used a virus‐induced gene silencing ( VIGS ) vector derived from the geminivirus Cabbage leaf curl virus (Ca LC uV) to assess natural variation in virus–host interactions in 190 Arabidopsis accessions. Silencing of CH ‐42 , encoding a protein needed to make chlorophyll, was used as a visible marker to discriminate asymptomatic accessions from those showing resistance. There was a wide range in symptom severity and extent of silencing in different accessions, but two correlations could be made. Lines with severe symptoms uniformly lacked extensive VIGS , and lines that showed attenuated symptoms over time (recovery) showed a concomitant increase in the extent of VIGS . One accession, Pla‐1, lacked both symptoms and silencing, and was immune to wild‐type infectious clones corresponding to Ca LC uV or Beet curly top virus ( BCTV ), which are classified in different genera in the Geminiviridae. It also showed resistance to the agronomically important Tomato yellow leaf curl virus ( TYLCV ). Quantitative trait locus mapping of a Pla‐1 X Col‐0 F 2 population was used to detect a major peak on chromosome 1, which is designated gip‐1 ( geminivirus immunity Pla‐1‐1 ). The recessive nature of resistance to Ca LC uV and the lack of obvious candidate genes near the gip‐1 locus suggest that a novel resistance gene(s) confers immunity.