2018 article

Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain

ADIPOSE-DERIVED STEM CELLS: METHODS AND PROTOCOLS, 2ND EDITION, Vol. 1773, pp. 215–230.

By: R. Nordberg  n, J. Bodle n & E. Loboa*

co-author countries: United States of America πŸ‡ΊπŸ‡Έ
author keywords: Adipose-derived stem cells; Tissue engineering; Mechanical stimulation; Tensile strain; Hydrostatic pressure; Microgravity; Differentiation; Osteogenesis; Chondrogenesis; Adipogenesis; Protocols; Methods
MeSH headings : Adipose Tissue / cytology; Cell Differentiation; Chondrogenesis; Humans; Hydrostatic Pressure; Mesenchymal Stem Cells / cytology; Musculoskeletal System; Osteogenesis; Primary Cell Culture; Stress, Mechanical; Tensile Strength; Tissue Engineering / methods; Weightlessness Simulation
Source: Web Of Science
Added: October 16, 2018

It is critical that human adipose stem cell (hASC) tissue-engineering therapies possess appropriate mechanical properties in order to restore function of the load bearing tissues of the musculoskeletal system. In an effort to elucidate the hASC response to mechanical stimulation and develop mechanically robust tissue engineered constructs, recent research has utilized a variety of mechanical loading paradigms including cyclic tensile strain, cyclic hydrostatic pressure, and mechanical unloading in simulated microgravity. This chapter describes methods for applying these mechanical stimuli to hASC to direct differentiation for functional tissue engineering of the musculoskeletal system.