2023 journal article
Scalable, Flexible, and Cost-Effective Seedling Grafting
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (191).
MeSH headings : Seedlings; Cost-Benefit Analysis; Arabidopsis / genetics; Plants; Solanum lycopersicum; Plant Roots
TL;DR:
A simple procedure to make an in-house reusable grafting device using silicone elastomer mix, and how to use this device for seedling grafting is described, and beginners can have their first successfully grafted seedlings in less than 3 weeks from start to finish.
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UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
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Source: Web Of Science
Added: May 30, 2023
Early-stage seedling grafting has become a popular tool in molecular genetics to study root-shoot relationships within plants. Grafting early-stage seedlings of the small model plant, Arabidopsis thaliana, is technically challenging and time consuming due to the size and fragility of its seedlings. A growing collection of published methods describe this technique with varying success rates, difficulty, and associated costs. This paper describes a simple procedure to make an in-house reusable grafting device using silicone elastomer mix, and how to use this device for seedling grafting. At the time of this publication, each reusable grafting device costs only $0.47 in consumable materials to produce. Using this method, beginners can have their first successfully grafted seedlings in less than 3 weeks from start to finish. This highly accessible procedure will allow plant molecular genetics labs to establish seedling grafting as a normal part of their experimental process. Due to the full control users have in the creation and design of these grafting devices, this technique could be easily adjusted for use in larger plants, such as tomato or tobacco, if desired.