2019 journal article
An In Vitro Assay of Disinfectants on the Viability of Heterakis gallinarum Eggs
Avian Diseases, 63(3), 511.
author keywords: Heterakis gallinarum; Histomonas meleagridis; in vitro assay; disinfectants; decontamination; nematode; embryonated eggs; trypan blue
MeSH headings : Animals; Antinematodal Agents / pharmacology; Ascaridida / drug effects; Disinfectants / pharmacology; In Vitro Techniques / methods; In Vitro Techniques / veterinary; Ovum / drug effects
TL;DR:
In a test of Clorox bleach, Green Klean, Decon7, Kem San, PLT, Virkon S, NaCl, dry limestone (CaCO3), and diesel fuel, only NaOCl (bleach) and Green K Lean damaged the eggshell, and only 20,625 ppm ofNaOCl rendered the larvae nonviable.
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2. Zero Hunger
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Added: January 17, 2020
SUMMARY. Nematodes are widespread and common in poultry. Disinfectants are used to reduce infection rates in poultry houses, but there is little documentation of their effectiveness. An in vitro assay was developed to test the efficacy of products to damage Heterakis gallinarum eggs, and nine disinfectants and chemicals commonly used in the poultry industry were tested. Embryonated eggs of H. gallinarum were pipetted into wells of plastic cell culture plates (250–300 eggs/well in water). Measured amounts of test articles were added to the suspensions for 2, 4, 6, or 24 hr. After exposure, eggs were washed with water and treated with trypan blue (1 ml of 0.4% solution, added to each well) at room temperature for 2 min. Eggshell integrity was determined microscopically by counting the number of eggs that were clear (intact) or that contained blue dye (compromised). As a test of embryo viability, five eggs per well from treatments containing compromised eggs were transferred to a Petri dish and hatched manually, using forceps to open the eggshell. Released larvae were then observed for signs of controlled movement. In a test of Clorox bleach (NaOCl), Green Klean, Decon7, Kem San, PLT, Virkon S, NaCl, dry limestone (CaCO3), and diesel fuel, only NaOCl (bleach) and Green Klean damaged the eggshell, and only 20,625 ppm of NaOCl rendered the larvae nonviable.