2020 journal article

An Efficient Stevia rebaudiana Transformation System and In vitro Enzyme Assays Reveal Novel Insights into UGT76G1 Function


By: Q. Wu n, C. La Hovary n, H. Chen n, X. Li n, H. Eng n, V. Vallejo*, R. Qu n, R. Dewey n

MeSH headings : Alternative Splicing; Glycosyltransferases / biosynthesis; Glycosyltransferases / genetics; Isoenzymes / biosynthesis; Isoenzymes / genetics; Plant Proteins / biosynthesis; Plant Proteins / genetics; Plants, Genetically Modified / enzymology; Plants, Genetically Modified / genetics; Stevia / enzymology; Stevia / genetics; Transformation, Genetic
TL;DR: A highly efficient Agrobacterium-mediated stable transformation system using axillary shoots as the initial explant is reported and evidence suggesting that alternative and/or aberrant splicing may serve to influence the ability of the plant to produce functional UGT76G1 transcripts, and possibly produce enzyme variants within the plant is found. (via Semantic Scholar)
Source: Web Of Science
Added: September 14, 2020