2021 journal article

Biosynthesis and characterization of deuterated chitosan in filamentous fungus and yeast


By: Y. Yuan n, H. Li*, W. Leite*, Q. Zhang*, P. Bonnesen*, J. Labbe*, K. Weiss*, S. Pingali* ...

author keywords: Microbial chitosan; Biodeuteration; Enzyme entrapment; NMR; Neutron scattering
MeSH headings : Biocompatible Materials / chemistry; Catalase; Chitosan / chemistry; Culture Media; Deuterium; Fungi / metabolism; Hydrogen / chemistry; Industrial Microbiology; Magnetic Resonance Spectroscopy; Rhizopus oryzae / metabolism; Saccharomycetales; Scattering, Small Angle; Spectroscopy, Fourier Transform Infrared
Source: Web Of Science
Added: March 15, 2021

Deuterated chitosan was produced from the filamentous fungus Rhizopus oryzae, cultivated with deuterated glucose in H2O medium, without the need for conventional chemical deacetylation. After extraction and purification, the chemical composition and structure were determined by Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and small-angle neutron scattering (SANS). 13C NMR experiments provided additional information about the position of the deuterons in the glucoseamine backbone. The NMR spectra indicated that the deuterium incorporation at the non-exchangeable hydrogen positions of the aminoglucopyranosyl ring in the C3 – C5 positions was at least 60–80 %. However, the C2 position was deuterated at a much lower level (6%). Also, SANS showed that the structure of deuterated chitosan was very similar compared to the non-deuterated counterpart. The most abundant radii of the protiated and deuterated chitosan fibers were 54 Å and 60 Å, respectively, but there is a broader distribution of fiber radii in the protiated chitosan sample. The highly deuterated, soluble fungal chitosan described here can be used as a model material for studying chitosan-enzyme complexes for future neutron scattering studies. Because the physical behavior of non-deuterated fungal chitosan mimicked that of shrimp shell chitosan, the methods presented here represent a new approach to producing a high quality deuterated non-animal-derived aminopolysaccharide for studying the structure-function association of biocomposite materials in drug delivery, tissue engineering and other bioactive chitosan-based composites.