2021 journal article
Developing mitochondrial DNA field-compatible tests
Critical Reviews in Environmental Science and Technology, 4, 1–41.
author keywords: Biosensors; environmental monitoring; isothermal amplification; mtDNA; nDNA; on-site field testing; paper-based assay
TL;DR:
Despite challenges, developing an integrated “sample-through detection” field test platform is the future direction for mtDNA molecular detection in environmental samples.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: April 21, 2021
Abstract Nucleic acid-based molecular detection of mitochondrial DNA (mtDNA) has been used for the detection of eukaryotic pathogens, genetic markers, and biomedical studies in humans and animals, forensic investigation, conservation biology, and environmental monitoring. Currently available molecular mtDNA testing is predominately dependent on relatively expensive laboratory equipment, which limits test applicability for on-site field testing. Recent technical advances in molecular testing of mtDNA using microfluidic chip technology and paper-based methods, and assays based on isothermal nucleic acid amplification show promise for field assay development. Microfluidic and paper-based components for mtDNA sample preparation and isothermal amplification establish the groundwork for targeted detection. The goal is on-site field detection mtDNA assays that combine sample processing, assay setup, and visual representation of the presence of mtDNA. This review examines advantages and disadvantages of alternative field-compatible tests for on-site detection of mtDNA. Despite challenges, developing an integrated “sample-through detection” field test platform is the future direction for mtDNA molecular detection in environmental samples. Graphical Abstract