2014 journal article

Effects of pentoxifylline on immediate and late-phase cutaneous reactions in response to anti-immunoglobulin E antibodies in clinically normal dogs

AMERICAN JOURNAL OF VETERINARY RESEARCH, 75(2), 152–160.

By: C. Pucheu-Haston*, K. Kasparek, R. Stout, M. Kearney & B. Hammerberg*

co-author countries: United States of America 🇺🇸
MeSH headings : Administration, Cutaneous; Animals; Antibodies, Anti-Idiotypic / adverse effects; Dog Diseases / chemically induced; Dog Diseases / drug therapy; Dogs; Free Radical Scavengers / therapeutic use; Immunoglobulin E / immunology; Inflammation / drug therapy; Inflammation / veterinary; Injections, Intradermal / veterinary; Intradermal Tests / veterinary; Pentoxifylline / therapeutic use; Skin Diseases / chemically induced; Skin Diseases / drug therapy; Skin Diseases / veterinary; Skin Tests / veterinary
Source: Web Of Science
Added: August 6, 2018

To characterize the effects of pentoxifylline on the gross and microscopic variables associated with immediate and late-phase inflammation following injection of IgE-specific antibodies in the skin of clinically normal dogs.6 healthy adult mixed-breed dogs.Intradermal injections (0.1 mL each) of PBS solution, histamine phosphate, and cross-linking rabbit-origin anti-canine IgE antibodies (3 injections/dog) were administered at 0 hours on day 0; wheal sizes were evaluated at 20 minutes, 6 hours, and 24 hours. Biopsy specimens of injected and noninjected skin were collected 24 hours after injection. On day 2, treatment with pentoxifylline (20 mg/kg, PO, q 8 h) was initiated and continued until day 30. For each dog, injection, measurement, and biopsy procedures were repeated on days 30 to 31 and on days 37 to 38 (ie, after discontinuation of pentoxifylline administration).Pentoxifylline administration was associated with a significant decrease in wheal size at 6 and 24 hours (but not at 20 minutes) after injection of anti-canine IgE. Repeated injections performed 1 week after drug discontinuation revealed partial recovery of the 6-hour cutaneous reaction and complete recovery of the 24-hour cutaneous reaction. Pentoxifylline administration was also associated with inhibition of mast cell degranulation and significant decreases in the total numbers of cutaneous inflammatory cells and eosinophils, compared with pretreatment findings.In clinically normal dogs, pentoxifylline effectively impaired late-phase reactions but not immediate reactions at sites of intradermal injection of IgE-specific antibodies by inhibiting mast cell degranulation and recruitment of cutaneous inflammatory cells, especially eosinophils.