2021 journal article
An optimized protocol for stepwise optimization of real-time RT-PCR analysis
Horticulture Research.
TL;DR:
This work proposed an optimized approach to sequentially optimizing primer sequences, annealing temperatures, primer concentrations, and cDNA concentration range for each reference (and target) gene and validated their utility under varying abiotic stress conditions.
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www.nature.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: ORCID
Added: August 1, 2021
Abstract