2022 journal article

Genomewide CRISPR knockout screen identified PLAC8 as an essential factor for SADS-CoVs infection

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 119(18).

By: L. Tse *, R. Meganck *, K. Araba*, B. Yount *, K. Shaffer *, Y. Hou *, J. Munt *, L. Adams * ...

author keywords: PLAC8; coronavirus; CRISPR; swine acute diarrhea syndrome coronavirus
MeSH headings : Alphacoronavirus / genetics; Animals; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; Coronavirus Infections / epidemiology; Swine; Swine Diseases
TL;DR: Using genomewide CRISPR knockout screening, the placenta-associated 8 protein (PLAC8) is identified as an essential host factor for SADS-CoV infection, uncovering a novel antiviral target for CoV infection. (via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being (Web of Science; OpenAlex)
Source: Web Of Science
Added: June 13, 2022

Significance A potential outbreak of swine acute diarrhea syndrome coronavirus (SADS-CoV) in the human population could be devastating. Using genomewide CRISPR knockout screening, we identified the placenta-associated 8 protein (PLAC8) as an essential host factor for SADS-CoV infection, uncovering a novel antiviral target for CoV infection. The PLAC8-related pathway may also have implications for other CoV infections. Given the ability of SADS-CoV to infect human primary cultures without adaptation, our findings lay the foundation for pandemic preparedness for the potential emergence of SADS-CoVs in response to the One Health Initiative.