2017 review

The evolution of chicken stem cell culture methods

[Review of ]. BRITISH POULTRY SCIENCE, 58(6), 681–686.

co-author countries: Iran (Islamic Republic of) 🇮🇷 United States of America 🇺🇸
author keywords: Chicken embryo; defined culture medium; stem cells; suspension culture; vaccine manufacture
MeSH headings : Animals; Cell Culture Techniques / methods; Cell Differentiation; Chickens; Embryonic Stem Cells / metabolism; Germ Cells / metabolism; Induced Pluripotent Stem Cells / metabolism
Source: Web Of Science
Added: August 6, 2018

1. The avian embryo is an excellent model for studying embryology and the production of pharmaceutical proteins in transgenic chickens. Furthermore, chicken stem cells have the potential for proliferation and differentiation and emerged as an attractive tool for various cell-based technologies. 2. The objective of these studies is the derivation and culture of these stem cells is the production of transgenic birds for recombinant biomaterials and vaccine manufacture, drug and cytotoxicity testing, as well as to gain insight into basic science, including cell tracking. 3. Despite similarities among the established chicken stem cell lines, fundamental differences have been reported between their culture conditions and applications. Recent conventional protocols used for expansion and culture of chicken stem cells mostly depend on feeder cells, serum-containing media and static culture. 4. Utilising chicken stem cells for generation of cell-based transgenic birds and a variety of vaccines requires large-scale cell production. However, scaling up the conventional adherent chicken stem cells is challenging and labour intensive. Development of a suspension cell culture process for chicken embryonic stem cells (cESCs), chicken primordial germ cells (PGCs) and chicken induced pluripotent stem cells (ciPSCs) will be an important advance for increasing the growth kinetics of these cells. 6. This review describes various approaches and suggestions to achieve optimal cell growth for defined chicken stem cells cultures and use in future manufacturing applications.