2022 journal article

High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes


author keywords: Transgene; Insertion site; Polyploid; T-DNA; Sequencing
MeSH headings : DNA, Bacterial / genetics; Plant Breeding; Plants, Genetically Modified / genetics; Transgenes
TL;DR: A simple method to identify the sites of transgene insertions using T-DNA-specific primers and high-throughput sequencing that enables identification of multiple insertion sites in the T 1 generation of any crop transformed via Agrobacterium. (via Semantic Scholar)
Source: Web Of Science
Added: October 17, 2022