High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes
BMC GENOMICS, 23(1).
author keywords: Transgene; Insertion site; Polyploid; T-DNA; Sequencing
MeSH headings : DNA, Bacterial / genetics; Plant Breeding; Plants, Genetically Modified / genetics; Transgenes
TL;DR:
A simple method to identify the sites of transgene insertions using T-DNA-specific primers and high-throughput sequencing that enables identification of multiple insertion sites in the T 1 generation of any crop transformed via Agrobacterium.
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