@article{grindem_breitschwerdt_perkins_cullins_thomas_hegarty_1999, title={Platelet-associated immunoglobulin (antiplatelet antibody) in canine Rocky Mountain spotted fever and ehrlichiosis}, volume={35}, ISSN={["0587-2871"]}, DOI={10.5326/15473317-35-1-56}, abstractNote={Antiplatelet antibodies were detected in the sera of dogs with naturally occurring and experimentally induced Rickettsia rickettsii and Ehrlichia canis infections. This is the first known report documenting elevated platelet-associated immunoglobulin (PAIg) titers in Rocky Mountain spotted fever (RMSF) infections. In the naturally occurring RMSF infections and ehrlichiosis, the antibodies persisted for weeks or months, even when the platelet counts had normalized. Results of this study indicate an immunological component for rickettsial thrombocytopenia. Therefore, current therapeutic recommendations, especially regarding avoiding the use of immunosuppressive drugs in patients with rickettsial diseases, need to be critically reviewed.}, number={1}, journal={JOURNAL OF THE AMERICAN ANIMAL HOSPITAL ASSOCIATION}, author={Grindem, CB and Breitschwerdt, EB and Perkins, PC and Cullins, LD and Thomas, TJ and Hegarty, BC}, year={1999}, pages={56–61} }
 @article{russell_perkins_hoffman_miller_walker_fuller_sellon_1999, title={Platelets from thrombocytopenic ponies acutely infected with equine infectious anemia virus are activated in vivo and hypofunctional}, volume={259}, ISSN={["0042-6822"]}, DOI={10.1006/viro.1999.9737}, abstractNote={Thrombocytopenia is a consistent finding and one of the earliest hematological abnormalities in horses acutely infected with equine infectious anemia virus (EIAV), a lentivirus closely related to human immunodeficiency virus. Multifactorial mechanisms, including immune-mediated platelet destruction and impaired platelet production, are implicated in the pathogenesis of EIAV-associated thrombocytopenia. This study was undertaken to investigate whether regenerative thrombopoiesis and platelet destruction occurred in ponies acutely infected with EIAV. Circulating large, immature platelets were increased in ponies acutely infected with EIAV late in the infection when platelet count was at a nadir. Morphometric analysis of bone marrow from acutely infected ponies revealed significant increased in megakaryocyte area and megakaryocyte nuclear area. A trend toward increased numbers of megakaryocytes was also observed. Platelets from acutely infected ponies had increased surface-bound fibrinogen and ultrastructural changes consistent with in vivo platelet activation. Platelets also had hypofunctional aggregation responses to three agonists in vitro. We conclude that thrombocytopenia in ponies acutely infected with EIAV is regenerative and suggest that bone marrow platelet production is not severely compromised in these ponies. Our findings reveal that in vivo platelet activation occurs in ponies acutely infected with EIAV, and as a result platelets are hypofunctional in vitro. Activation of platelets in vivo may cause platelet degranulation or formation of platelet aggregates, which would result in removal of these damages platelets from circulation. This may represent a form of nonimmune-mediated platelet destruction in ponies acutely infected with EIAV.}, number={1}, journal={VIROLOGY}, author={Russell, KE and Perkins, PC and Hoffman, MR and Miller, RT and Walker, KM and Fuller, FJ and Sellon, DC}, year={1999}, month={Jun}, pages={7–19} }
 @article{russell_perkins_grindem_walker_sellon_1997, title={Flow cytometric method for detecting thiazole orange positive (reticulated) platelets in thrombocytopenic horses}, volume={58}, number={10}, journal={American Journal of Veterinary Research}, author={Russell, K. E. and Perkins, P. C. and Grindem, C. B. and Walker, K. M. and Sellon, D. C.}, year={1997}, pages={1092–1096} }
 @article{perkins_grindem_carter_1996, title={Flow cytometry: The next step in diagnostic testing}, volume={18}, number={4}, journal={Compendium on Continuing Education for the Practicing Veterinarian}, author={Perkins, P. C. and Grindem, C. B. and Carter, P. B.}, year={1996}, pages={421} }
 @article{perkins_grindem_1995, title={Evaluation of six cytometric methods for reticulocyte enumeration and differentiation in the cat}, volume={24}, number={2}, journal={Veterinary Clinical Pathology}, author={Perkins, P. C. and Grindem, C. B.}, year={1995}, pages={37} }
 @article{perkins_grindem_cullins_1995, title={Flow cytometric analysis of punctate and aggregate reticulocyte responses in phlebotomized cats}, volume={56}, number={12}, journal={American Journal of Veterinary Research}, author={Perkins, P. C. and Grindem, C. B. and Cullins, L. D.}, year={1995}, pages={1564} }
 @article{perkins_grindem_levy_1995, title={What is your diagnosis? An 11-year-old domestic longhaired cat with anemia}, volume={24}, number={3}, journal={Veterinary Clinical Pathology}, author={Perkins, P. C. and Grindem, C. B. and Levy, J. K.}, year={1995}, pages={77} }