Thomas J. Phelan

Works (1)

Updated: July 5th, 2023 15:59

2003 article

Elevation of Transgene Expression Level by Flanking Matrix Attachment Regions (MAR) is Promoter Dependent: A Study of the Interactions of Six Promoters with the RB7 3′ MAR

Mankin, S. L., Allen, G. C., Phelan, T., Spiker, S., & Thompson, W. F. (2003, February 1). Transgenic Research, Vol. 12, pp. 3–12.

By: S. Mankin n, G. Allen*, T. Phelan n, S. Spiker n & W. Thompson n 

author keywords: gene silencing; matrix attachment regions; transformation; transgene expression
MeSH headings : 3' Untranslated Regions / genetics; DNA Primers / chemistry; Gene Expression Regulation, Plant; Genes, Plant / physiology; Glucuronidase / genetics; Glucuronidase / metabolism; Hot Temperature; Nuclear Matrix / physiology; Plant Leaves / genetics; Plant Leaves / growth & development; Plants, Genetically Modified / genetics; Plants, Genetically Modified / growth & development; Polymerase Chain Reaction; Promoter Regions, Genetic / genetics; Tobacco / genetics; Tobacco / growth & development; Transformation, Genetic; Transgenes / physiology; Up-Regulation
topics (OpenAlex): Transgenic Plants and Applications; Plant tissue culture and regeneration; Plant Virus Research Studies
TL;DR: Most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs, which is important for understanding the role of matrix attachment region in transgene expression. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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