@article{ferguson_tekrony_egli_1990, title={CHANGES DURING EARLY SOYBEAN SEED AND AXES DETERIORATION .1. SEED QUALITY AND MITOCHONDRIAL RESPIRATION}, volume={30}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1990.0011183X003000010038x}, abstractNote={Understanding the cause of soybean [Glycine max (L.) Merr.] seed deterioration during storage will assist in the development of accurate laboratory tests to evaluate seed vigor. This study was designed to investigate the changes that occur in mitochondria! respiration during the early stages of soybean seed deterioration. High‐quality seed lots of ‘Desoto’ and ‘Union’ soybean were stored at 25 °C and 115 g kg‐1 seed moisture. Seed quality (germination and vigor) and conductivity of leachate from embryonic axes were measured at monthly intervals during storage. Mitochondrial State 3 (ST 3) respiration rates and respiratory control ratios (RCR) were measured from excised axes of samples after 24 h of imbibition. Germination remained high for seeds of both cultivars throughout storage; however, seed vigor (as measured by accelerated aging) declined significantly after 7 and 10 months for Union and Desoto, respectively. Conductivity of the leachate from excised axes for both cultivars increased early during storage prior to detectable changes in whole seed vigor. State 3 respiration rates of mitochondria from axes decreased throughout storage and RCR values declined after 2 months for Union and after 4 months for Desoto. Decreases in ST 3 respiration rates and RCR in both cultivars preceded measurable declines in whole‐seed vigor and correlated with increases in the conductivity of axes leachate.}, number={1}, journal={CROP SCIENCE}, author={FERGUSON, JM and TEKRONY, DM and EGLI, DB}, year={1990}, pages={175–179} }
 @article{ferguson_tekrony_egli_1990, title={CHANGES DURING EARLY SOYBEAN SEED AND AXES DETERIORATION .2. LIPIDS}, volume={30}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci1990.0011183X003000010039x}, abstractNote={Peroxidative changes in seed lipids are thought to be a major cause of seed deterioration during storage. This study was conducted to determine the fatty‐acid composition of soybean [Glycine max (L.) Merr.] seeds, axes, and mitochondria during the early stages of deterioration. High‐quality seed lots of ‘Union’ and ‘Desoto’ soybean were stored at 25 °C and 115 g kg‐1 seed moisture for 10 and 12 months, respectively. Seeds were sampled at frequent intervals and the fatty‐acid composition of storage and membrane lipids from seeds, axes, and mitochondria were determined. There were no detectable changes in the fatty‐acid composition of storage lipids from whole seeds or axes during the storage period for either seed lot, despite the substantial decline in seed vigor. There was a slight decrease in the percentage of unsaturated fatty acids in the membrane lipids of excised axes from Desoto seeds. The percentage of unsaturated fatty acids in the mitochondria from excised axes declined early during storage. The double‐bond index (DBI) of lipids from mitochondria of the Union axes fell from 1.76 at the beginning of storage to 1.60 after 10 months. Similar declines of 1.79 to 1.70 after 1 yr in storage were seen for the Desoto axes. Mitochondria! respiration and DBI were highly correlated for both seed lots. These data suggest that the decreases in mitochondrial respiration during storage may be associated with peroxidative changes in mitochondrial lipids and that these changes occurred prior to loss in seed vigor.}, number={1}, journal={CROP SCIENCE}, author={FERGUSON, JM and TEKRONY, DM and EGLI, DB}, year={1990}, pages={179–182} }