1992 journal article

Development and characterization of a generalized gene tagging system for higher plants using an engineered maize transposon Ac

Plant Molecular Biology, 20(2), 177–198.

By: W. Fitzmaurice n, L. Lehman n, L. Nguyen n, W. Thompson n, E. Wernsman n & M. Conkling n

author keywords: AC; DS; BETA-GLUCURONIDASE; METHOTREXATE RESISTANCE; TOBACCO; TRANSPOSON TAGGING
MeSH headings : Base Sequence; Blotting, Southern; Cloning, Molecular / methods; Crosses, Genetic; DNA; DNA Transposable Elements; Drug Resistance / genetics; Genetic Engineering; Genetic Markers; Glucuronidase / genetics; Methotrexate / pharmacology; Molecular Sequence Data; Nucleotidyltransferases / genetics; Nucleotidyltransferases / metabolism; Plants / genetics; Plants, Toxic; Polymerase Chain Reaction; Promoter Regions, Genetic; Rhizobium; Seeds / genetics; Tobacco; Transformation, Genetic; Transposases; Zea mays / genetics
TL;DR: This binary system includes the transposase (Ts) and the tagging element (Ds) on separate T-DNA vectors for dicotyledonous plants and can generate a sufficient number of events to be useful in gene tagging. (via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger (OpenAlex)
Sources: Web Of Science, Crossref, NC State University Libraries
Added: August 6, 2018

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