W. P. Fitzmaurice
Works (1)
Updated: July 5th, 2023 15:53
1992 journal article
Development and characterization of a generalized gene tagging system for higher plants using an engineered maize transposon Ac
Plant Molecular Biology, 20(2), 177–198.
author keywords: AC; DS; BETA-GLUCURONIDASE; METHOTREXATE RESISTANCE; TOBACCO; TRANSPOSON TAGGING
MeSH headings : Base Sequence; Blotting, Southern; Cloning, Molecular / methods; Crosses, Genetic; DNA; DNA Transposable Elements; Drug Resistance / genetics; Genetic Engineering; Genetic Markers; Glucuronidase / genetics; Methotrexate / pharmacology; Molecular Sequence Data; Nucleotidyltransferases / genetics; Nucleotidyltransferases / metabolism; Plants / genetics; Plants, Toxic; Polymerase Chain Reaction; Promoter Regions, Genetic; Rhizobium; Seeds / genetics; Tobacco; Transformation, Genetic; Transposases; Zea mays / genetics
TL;DR:
This binary system includes the transposase (Ts) and the tagging element (Ds) on separate T-DNA vectors for dicotyledonous plants and can generate a sufficient number of events to be useful in gene tagging.
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UN Sustainable Development Goal Categories
2. Zero Hunger
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3. Good Health and Well-being
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Added: August 6, 2018