@article{jin_petters_im_1994, title={Transgenic livestock}, volume={7}, DOI={10.5713/ajas.1994.1}, number={1}, journal={Asian-Australasian Journal of Animal Sciences}, author={Jin, D. I. and Petters, R. M. and Im, K. S.}, year={1994}, pages={1} } @article{jin_petters_johnson_shuman_1991, title={SURVIVAL OF EARLY PREIMPLANTATION PORCINE EMBRYOS AFTER COCULTURE WITH CELLS PRODUCING AN AVIAN RETROVIRUS}, volume={35}, ISSN={["1879-3231"]}, DOI={10.1016/0093-691X(91)90448-M}, abstractNote={To determine the relative survival of porcine embryos after co-culture with cells producing an avian retrovirus, four-cell stage embryos were obtained from sows following synchronization with altrenogest and superovulation with gonadotropins. These embryos were randomly assigned to the following treatments: no manipulation (zona-intact); zona removed with acidified Tyrode's solution (zona-free); and zona removed followed by co-culture with D-17 canine cells producing an avian retrovirus vector derived from spleen necrosis virus (zona-free + co-culture). The survival rates of four-cell stage embryos to morulae or early blastocysts during a 48-h culture period were 93.3, 80.0 and 57.7% in zona-intact, zona-free and zona-free + co-culture groups, respectively. Following embryo transfer, the development of embryos to fetuses at six weeks of gestation was 37.5, 30.0 and 11.7% in zona-intact, zona-free and zona-free + co-culture groups. These results indicate that early preimplantation porcine embryos can develop to apparently normal fetuses following co-culture with cells producing a retrovirus, and the feasibility of this method for retrovirus-mediated gene transfer in pigs was demonstrated.}, number={3}, journal={THERIOGENOLOGY}, author={JIN, DI and PETTERS, RM and JOHNSON, BH and SHUMAN, RM}, year={1991}, month={Mar}, pages={521–526} }