@article{reed_wernsman_burns_kramer_1988, title={AN EVALUATION OF THE USE OF IRRADIATED POLLEN FOR GENE-TRANSFER IN NICOTIANA}, volume={56}, ISSN={["0168-9452"]}, DOI={10.1016/0168-9452(88)90029-5}, abstractNote={The use of irradiated pollen to effect ‘egg-transformation’ in Nicotiana was tested using a nuclear chlorophyll-deficiency marker. Yellow-green (yg) plants of Nicotiana tabacum were pollinated with pollen from N. glutinosa which had received 50, 75, or 100 krads of gamma-irradiation. Progeny were scored for leaf color and chromosome number. Only one possible transformant was recovered; however, upon selfing, this plant segregated for leaf color. Thus, it could not be determined if this plant was the result of contaminant pollination, mutation, or a transformation event that occurred after diploidization. All other dark green plants had at least one intact chromosome from N. glutinosa. While no evidence in support of the egg-transformation theory was obtained from this study, cytological data concerning the use of irradiated pollen for haploid induction and for the rapid production of alien chromosome addition lines was presented.}, number={2}, journal={PLANT SCIENCE}, author={REED, SM and WERNSMAN, EA and BURNS, JA and KRAMER, MG}, year={1988}, pages={155–160} }