@article{walsh_swaisgood_1996, title={Investigating the use of the chymosin-sensitive sequence of kappa-casein as a cleavable linker site in fusion proteins}, volume={45}, ISSN={["0168-1656"]}, DOI={10.1016/0168-1656(95)00178-6}, abstractNote={The chymosin-sensitive sequence of bovine k-casein A (kappa-CN A) was investigated as a cleavable linker site between the two domains of a streptavidin-chloramphenicol acetyltransferase fusion protein. Two DNA sequences were synthesized which encode the amino acids from 101 to 107 and from 97 to 113 of bovine kappa-CN A. These sequences were separately cloned in-frame to a streptavidin expression vector used for fusion protein construction. The gene for chloramphenicol acetyltransferase (CAT) was then cloned in-frame to a streptavidin-chymosin-sensitive linker vector forming plasmids pStCL1CAT and pStCL2CAT. The fusion protein was expressed in Escherichia coli and SDS-PAGE and Western blot analysis of chymosin-treated cell lysates showed a pH-dependent cleavage of the fusion proteins. Fusion proteins were also bioselectively immobilized onto biotinylated controlled-pore glass beads and treated with chymosin. CAT was specifically released by chymosin treatment and was identified by SDS-PAGE.}, number={3}, journal={JOURNAL OF BIOTECHNOLOGY}, author={Walsh, MK and Swaisgood, HE}, year={1996}, month={Mar}, pages={235–241} }
 @article{walsh_swaisgood_1991, title={Preliminary studies of beta-galactosidase bioreactors constructed by biospecific adsorption of streptavidin-enzyme conjugates or streptavidin-enzyme fusion proteins}, volume={74}, journal={Journal of Dairy Science}, author={Walsh, M. K. and Swaisgood, H. E.}, year={1991}, pages={88} }