@article{wolff_abdelaal_goh_lampert_roe_1994, title={ORGANOPHOSPHATE RESISTANCE IN THE TOBACCO APHID (HOMOPTERA, APHIDIDAE) - PURIFICATION AND CHARACTERIZATION OF A RESISTANCE-ASSOCIATED ESTERASE}, volume={87}, ISSN={["0022-0493"]}, DOI={10.1093/jee/87.5.1157}, abstractNote={A 1-naphthyl acetate esterase was identified by isoelectric focusing in organophosphate resistant (red) tobacco aphids, Myzus nicotianae Blackman, which apparently was lacking in susceptible (green) individuals of the same species. This resistance-associated esterase (RAE) was purified from the cytosol of whole body homogenates of adult tobacco aphids by gel permeation chromatography and two successive DEAE-Sephacel ion exchange columns. The enzyme eluted from the second DEAE column in 0.23 to 0.28 M NaCl and was ≍95% pure as determined by high-performance liquid chromatography (HPLC) on a BIO-SIL TSK-125 column. Purified RAE had an apparent molecular weight of 67,300 as determined by gel permeation HPLC and 66,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Wide range isoelectric focusing (pH 3.5-9.5) on polyacrylamide gel plates indicated that the isoelectric point was 4.61. Rabbit antiserum to the green peach aphid, M. persicae (Sulzer), esterase (E4) specifically cross-reacted with the tobacco aphid RAE, and the two esterases had identical molecular weights and isoelectric points as determined by immunoblotting. RAE protein was not detectable in susceptible tobacco aphids.}, number={5}, journal={JOURNAL OF ECONOMIC ENTOMOLOGY}, author={WOLFF, MA and ABDELAAL, YAI and GOH, DKS and LAMPERT, EP and ROE, RM}, year={1994}, month={Oct}, pages={1157–1164} }