@article{ziecik_esbenshade_howard_britt_1990, title={ONTOGENY OF THE GONADAL RECEPTOR FOR LUTEINIZING-HORMONE IN THE PIG}, volume={33}, ISSN={["0093-691X"]}, DOI={10.1016/0093-691X(90)90535-2}, abstractNote={Homogenates of porcine ovaries and testes collected between 70 d post coitum and 42 d post partum were incubated with radiolabelled human chorionic gonadotropin (hCG) to determine the presence and relative amounts of luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptors. Specific binding of 125I-hCG to ovaries and testes occurred at all stages of fetal and postnatal development. Ovarian tissue possessed relatively low affinity, high capacity LH/hCG binding sites that were most numerous at Day 80 of gestation and decreased thereafter. In contrast, high affinity, low capacity LH/hCG binding sites were found in the testes. In males, the total number of LH/hCG binding sites remained stable until near term and then increased with age, but the number of sites per gram of testicular tissue did not change (P>0.05). In summary, differential binding of LH/hCG in gonadal tissue occurred in male and female piglets during pre- and post-natal periods, and this binding reflected the known differential pattern of development of the male and female gonad.}, number={3}, journal={THERIOGENOLOGY}, author={ZIECIK, AJ and ESBENSHADE, KL and HOWARD, HJ and BRITT, JH}, year={1990}, month={Mar}, pages={583–589} }
 @article{ziecik_esbenshade_howard_britt_1990, title={SEX-RELATED DIFFERENCES IN THE CONTROL OF GONADOTROPIN CONCENTRATIONS IN NEONATAL PIGS}, volume={23}, ISSN={["0378-4320"]}, DOI={10.1016/0378-4320(90)90054-J}, abstractNote={Two experiments were conducted to examine gonadal regulation of gonadotropin secretion in male and female neonatal piglets. In the first experiment, a single i.p. injection of charcoal-treated porcine follicular fluid (pFF) lowered (P < 0.05) concentrations of plasma follicle-stimulating hormone (FSH) at 6 and 12 h after administration in 7-day-old intact male piglets, but failed to alter FSH in castrated male or intact female piglets. In the second experiment, administration of pFF twice daily from day 0 to 14 of life caused an increase in plasma FSH levels in intact males, but did not significantly alter FSH in females or in males castrated at birth. Plasma LH did not differ between male and female piglets, but FSH was significantly lower in males than in females. Castration of males at birth resulted in lower testosterone and estradiol-17β levels and resulted in a gradual increase in concentrations of FSH over the 14-day experimental period. Treatment of neonatal pigs with pFF altered concentrations of FSH in intact males, but not in intact females, indicating a divergence between the sexes in the postnatal control of FSH secretion.}, number={2}, journal={ANIMAL REPRODUCTION SCIENCE}, author={ZIECIK, AJ and ESBENSHADE, KL and HOWARD, HJ and BRITT, JH}, year={1990}, month={Sep}, pages={123–133} }