Ko-yi Chien

Works (4)

Updated: July 5th, 2023 15:50

2013 article

Autophosphorylation of gatekeeper tyrosine by symbiosis receptor kinase

Samaddar, S., Dutta, A., Sinharoy, S., Paul, A., Bhattacharya, A., Saha, S., … DasGupta, M. (2013, August 17). FEBS Letters.

By: S. Samaddar *, A. Dutta*, S. Sinharoy *, A. Paul *, A. Bhattacharya*, S. Saha *, K. Chien n, M. Goshe n, M. DasGupta *

author keywords: Autophosphorylation; Gatekeeper residue; Receptor-like kinase; Symbiosis receptor kinase; Tyrosine kinase-like
MeSH headings : Amino Acid Motifs; Arachis / genetics; Arachis / metabolism; Catalytic Domain; Cell Line; Gene Expression Regulation, Plant; Mutation; Phosphorylation; Plant Proteins / genetics; Plant Proteins / metabolism; Protein Binding; Receptor Protein-Tyrosine Kinases / genetics; Receptor Protein-Tyrosine Kinases / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Signal Transduction; Tyrosine / genetics; Tyrosine / metabolism
topics (OpenAlex): Legume Nitrogen Fixing Symbiosis; Plant-Microbe Interactions and Immunity; Plant Pathogenic Bacteria Studies
TL;DR: It is hypothesize gatekeeper phosphorylation to be associated with synthesis/maturation of AhSYMRK and this phenomenon may be prevalent among RLKs. (via Semantic Scholar)
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Added: August 6, 2018

2012 article

Proteomic and Phosphoproteomic Analysis of Chicken Embryo Fibroblasts Infected with Cell Culture-Attenuated and Vaccine Strains of Marek’s Disease Virus

Chien, K.-yi, Blackburn, K., Liu, H.-C., & Goshe, M. B. (2012, October 29). Journal of Proteome Research.

By: K. Chien n, K. Blackburn n, H. Liu n & M. Goshe n

author keywords: proteomics; phosphoproteomics; liquid chromatography; mass spectrometry; Marek's Disease Virus; phosphorylation
MeSH headings : Amino Acid Sequence; Animals; Cells, Cultured; Chick Embryo; Chromatography, Affinity / methods; Eukaryotic Initiation Factor-4E / metabolism; Fibroblasts / metabolism; Fibroblasts / virology; Herpesvirus 2, Gallid / classification; Herpesvirus 2, Gallid / pathogenicity; Marek Disease; Mass Spectrometry / methods; Molecular Sequence Data; Multiprotein Complexes / analysis; Multiprotein Complexes / metabolism; Peptide Chain Initiation, Translational; Phosphopeptides / analysis; Phosphopeptides / metabolism; Phosphoproteins / analysis; Phosphoproteins / metabolism; Phosphorylation; Proteome / analysis; Proteome / metabolism; Proteomics / methods; Transcription, Genetic; Viral Plaque Assay
topics (OpenAlex): Herpesvirus Infections and Treatments; Poxvirus research and outbreaks; Cytomegalovirus and herpesvirus research
TL;DR: A kinase-dependent pathway to regulate phosphorylation of 4E-BP1 to modulate assembly of the protein translation initiation complex was revealed and provided new insight for future functional and biochemical characterization of specific proteins involved in MDV pathogenesis. (via Semantic Scholar)
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Added: August 6, 2018

2011 article

Development and Application of a Phosphoproteomic Method Using Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC), IMAC, and LC–MS/MS Analysis to Study Marek’s Disease Virus Infection

Chien, K.-yi, Liu, H.-C., & Goshe, M. B. (2011, July 8). Journal of Proteome Research.

By: K. Chien n, H. Liu n & M. Goshe n

author keywords: phosphoproteomics; liquid chromatography; mass spectrometry; Marek's Disease Virus; phosphorylation
MeSH headings : Amino Acid Sequence; Animals; Avian Proteins / analysis; Avian Proteins / metabolism; Chick Embryo; Chromatography, Liquid; Fibroblasts; Host-Pathogen Interactions / physiology; Hydrophobic and Hydrophilic Interactions; Mardivirus; Marek Disease / immunology; Marek Disease / metabolism; Marek Disease / virology; Molecular Sequence Data; Peptide Fragments / analysis; Peptide Fragments / metabolism; Phosphopeptides / analysis; Phosphopeptides / metabolism; Proteomics / methods; Proteomics / standards; Reproducibility of Results; Static Electricity; Tandem Mass Spectrometry; Viral Proteins / analysis; Viral Proteins / metabolism
topics (OpenAlex): Herpesvirus Infections and Treatments; Cytomegalovirus and herpesvirus research; Yersinia bacterium, plague, ectoparasites research
TL;DR: An effective separation and enrichment approach is developed to investigate the changes occurring in the phosphoproteome using electrostatic repulsion-hydrophilic interaction chromatography to fractionate peptides from chicken embryo fibroblast (CEF) digests and incorporating a subsequent IMAC enrichment step to selectively target phosphorylated peptides for LC-MS/MS analysis. (via Semantic Scholar)
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2009 article

Advances in Quantitative Mass Spectrometry Analysis: Weighing in on Isotope Coding and Label-Free Approaches for Expression and Functional Proteomics

Chien, K.-yi, & Goshe, M. (2009, April 1). Current Analytical Chemistry.

By: K. Chien* & M. Goshe*

author keywords: Mass spectrometry; Quantitative proteomics; Phosphoproteomics; Isotope coding; Label-free quantitation; Absolute quantitation
topics (OpenAlex): Advanced Proteomics Techniques and Applications; Mass Spectrometry Techniques and Applications; Metabolomics and Mass Spectrometry Studies
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Added: August 6, 2018

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