@article{rinker_han_kelly_1999, title={Continuous culture as a tool for investigating the growth physiology of heterotrophic hyperthermophiles and extreme thermoacidophiles}, volume={85}, number={1999}, journal={Journal of Applied Microbiology}, author={Rinker, K. D. and Han, C. J. and Kelly, R. M.}, year={1999}, pages={118S–127} }
 @article{han_kelly_1998, title={Biooxidation capacity of the extremely thermoacidophilic archaeon Metallosphaera sedula under bioenergetic challenge}, volume={58}, ISSN={["0006-3592"]}, DOI={10.1002/(SICI)1097-0290(19980620)58:6<617::AID-BIT7>3.0.CO;2-L}, abstractNote={The biooxidation capacity of an extremely thermoacidophilic archaeon Metallosphaera sedula (DSMZ 5348) was examined under bioenergetic challenges imparted by thermal or chemical stress in regard to its potential use in microbial bioleaching processes. Within the normal growth temperature range of M. sedula (70-79 degrees C) at pH 2.0, upward temperature shifts resulted in bioleaching rates that followed an Arrhenius-like dependence. When the cells were subjected to supraoptimal temperatures through gradual thermal acclimation at 81 degrees C (Han et al., 1997), cell densities were reduced but 3 to 5 times faster specific leaching rates (Fe3+ released from iron pyrite/cell/h) could be achieved by the stressed cells compared to cells at 79 degrees C and 73 degrees C, respectively. The respiration capacity of M. sedula growing at 74 degrees C was challenged by poisoning the cells with uncouplers to generate chemical stress. When the protonophore 2,4-dinitrophenol (5-10 μM) was added to a growing culture of M. sedula on iron pyrite, there was little effect on specific leaching rates compared to a culture with no protonophore at 74 degrees C; 25 μM levels proved to be toxic to M. sedula. However, a significant stimulation in specific rate was observed when the cells were subjected to 1 μM nigericin (+135%) and 2 μM (+63%); 5 μM levels of the ionophore completely arrested cell growth. The ionophore effect was further investigated in continuous culture growing on ferrous sulfate at 74 degrees C. When 1 μM nigericin was added as a pulse to a continuous culture, a 30% increase in specific iron oxidation rate was observed for short intervals, indicating a potential positive impact on leaching when periodic chemical stress is applied. This study suggests that biooxidation rates can be increased by strategic exposure of extreme thermoacidophiles to chemical or thermal stress, and this approach should be considered for improving process performance. Copyright 1998 John Wiley & Sons, Inc.}, number={6}, journal={BIOTECHNOLOGY AND BIOENGINEERING}, author={Han, CJ and Kelly, RM}, year={1998}, month={Jun}, pages={617–624} }
 @article{karve_han_rizwan-uddin_1998, title={Improved axial flux shape generator for a quick departure from nucleate boiling test}, volume={123}, ISSN={["0029-5450"]}, DOI={10.13182/NT98-A2886}, abstractNote={Axial power shapes that develop during power-maneuvering simulations in pressurized water reactors must be analyzed to ensure that an adequate margin to avoid departure from nucleate boiling (DNB) is maintained during these transients. To reduce the number of flux shapes that need to be analyzed in detail to determine the DNB ratio (DNBR), often generic axial flux shapes are analyzed and maximum-allowable-peaking (MAP) limits are determined to conservatively filter those actual axial power shapes that are clearly safe. Current generic MAP limits, obtained for axial flux shapes generated by a two-parameter-based axial flux shape generator, are overly conservative for some power shapes and are nonconservative for others, leading to unnecessary operational restrictions on conservative cases. A penalty is imposed on nonconservative cases. To reduce the number of overly conservative and nonconservative cases, a new generic axial power shape generator that is based on three parameters is developed. Generic MAP limits have been developed for the new axial flux shape generator and tested using real flux shapes by plotting the percent deviation ofMAP limits for generic flux shapes from the corresponding value for actual flux shapes. A new axial flux shape generator, which is clearly superior because it leads to a significantly lower percent deviation, will lead to reduced man-hours for detailed DNBR analyses and remove some of the unnecessary operational restrictions imposed by the old flux shape generator.}, number={2}, journal={NUCLEAR TECHNOLOGY}, author={Karve, AA and Han, C and Rizwan-Uddin}, year={1998}, month={Aug}, pages={121–129} }
 @article{han_park_kelly_1997, title={Acquired thermotolerance and stressed-phase growth of the extremely thermoacidophilic Archaeon Metallosphaera sedula in continuous culture}, volume={63}, number={6}, journal={Applied and Environmental Microbiology}, author={Han, C. J. and Park, S. H. and Kelly, R. M.}, year={1997}, pages={2391–2396} }