Marilyn Ehrenshaft
Works (21)
Updated: July 5th, 2023 16:03
2004 journal article
A highly conserved gene for vitamin B-6 biosynthesis may have consequences for stress and hormone responses in plants
Physiologia Plantarum, 121(1), 14-.
Source: NC State University Libraries
Added: August 6, 2018
2004 journal article
Functional complementation between the PDX1 vitamin B-6 biosynthetic gene of Cercospora nicotianae and pdxJ of Escherichia coli
FEBS LETTERS, 564(1-2), 143–146.
author keywords: SNZ; YaaD; SOR1; PYROA; pyridoxal-5 '-phosphate; pyridoxamine
MeSH headings : Ascomycota / genetics; Cloning, Molecular; Enzymes / physiology; Escherichia coli Proteins / genetics; Escherichia coli Proteins / physiology; Fungal Proteins / genetics; Fungal Proteins / physiology; Genes, Fungal; Genetic Complementation Test; Ligases / genetics; Ligases / physiology; Oxidoreductases; Pyridoxine / biosynthesis; Transformation, Genetic; Vitamin B 6 / biosynthesis
TL;DR:
The PDX1 gene of the alternative B6 pathway encodes a protein responsible for synthesis of the pyridoxine ring, which is likely to be responsible for de novo vitamin B6 biosynthesis in Escherichia coli.
(via Semantic Scholar)
febs.onlinelibrary.wiley.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2003 journal article
Cercosporin-deficient mutants by plasmid tagging in the asexual fungus Cercospora nicotianae
MOLECULAR GENETICS AND GENOMICS, 270(2), 103–113.
author keywords: photosensitizer; plasmid rescue; active oxygen
MeSH headings : Amino Acid Sequence; Ascomycota / genetics; Ascomycota / metabolism; Ascomycota / pathogenicity; Base Sequence; Chromosome Mapping; DNA, Fungal / genetics; Gene Targeting; Genetic Vectors; Molecular Sequence Data; Multienzyme Complexes / genetics; Multienzyme Complexes / metabolism; Mutation; Mycotoxins / biosynthesis; Mycotoxins / genetics; Perylene / analogs & derivatives; Perylene / metabolism; Phenotype; Pigmentation; Plasmids / genetics; Sequence Homology, Amino Acid; Transformation, Genetic
TL;DR:
The adaptation of plasmid insertion and restriction enzyme-mediated integration to produce cercosporin toxin-deficient mutants in the asexual phytopathogenic fungus Cercospora nicotianae is successfully adapted, providing the first molecular evidence that cercOSporin is synthesized via a polyketide pathway as previously hypothesized.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2003 journal article
Expression of the cercosporin toxin resistance gene (CRG1) as a dicistronic mRNA in the filamentous fungus Cercospora nicotianae
CURRENT GENETICS, 43(6), 415–424.
author keywords: photosensitizer; singlet oxygen; toxin; transcription factor; uracil transporter; zinc finger DNA-binding domain
MeSH headings : Amino Acid Sequence; Ascomycota / drug effects; Ascomycota / genetics; Ascomycota / metabolism; Base Sequence; Drug Resistance / genetics; Fungal Proteins / biosynthesis; Fungal Proteins / genetics; Gene Expression; Genes; Genome, Fungal; Molecular Sequence Data; Nucleotide Transport Proteins / genetics; Nucleotide Transport Proteins / metabolism; Perylene / analogs & derivatives; Perylene / pharmacology; RNA, Fungal / metabolism; RNA, Messenger / metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transcription Factors / genetics; Transcription Factors / metabolism; Transcription, Genetic
TL;DR:
It is concluded that the 4.5-kb transcript is a dicistronic mRNA of both CRG1 and PUT1 in the fungus C. nicotianae, the first example of a d physicistronic mRNA identified in filamentous fungi.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Functional expression and cellular localization of cercosporin-resistance proteins fused with the GFP in Cercospora nicotianae
CURRENT GENETICS, 41(3), 159–167.
author keywords: photosensitizer; singlet oxygen; fungi; pyridoxine
MeSH headings : Ascomycota / drug effects; Ascomycota / genetics; Ascomycota / metabolism; Base Sequence; Blotting, Western; DNA Primers; Fungal Proteins / genetics; Fungal Proteins / metabolism; Green Fluorescent Proteins; Luminescent Proteins / metabolism; Microscopy, Confocal; Perylene / analogs & derivatives; Perylene / pharmacology; Recombinant Fusion Proteins / genetics; Recombinant Fusion Proteins / metabolism; Transformation, Genetic
TL;DR:
Transformation of these constructs into strains mutant in these respective genes resulted in green-fluorescent transformants complemented for the mutant phenotype, a result inconsistent with microscopic observation and computer sequence analysis, which suggests that CRG1 is a membrane protein.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2002 journal article
Sugar alcohols, salt stress, and fungal resistance: Polyols - Multifunctional plant protection?
Journal of the American Society for Horticultural Science, 127(4), 467–473.
Source: NC State University Libraries
Added: August 6, 2018
2001 journal article
Isolation of PDX2, a second novel gene in the pyridoxine biosynthesis pathway of eukaryotes, archaebacteria, and a subset of eubacteria
JOURNAL OF BACTERIOLOGY, 183(11), 3383–3390.
MeSH headings : Amino Acid Sequence; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Ascomycota / genetics; Ascomycota / metabolism; Bacillus subtilis / genetics; Bacillus subtilis / metabolism; Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Base Sequence; Fungal Proteins / genetics; Fungal Proteins / isolation & purification; Fungal Proteins / metabolism; Gene Targeting; Genetic Complementation Test; Molecular Sequence Data; Mutation; Mycobacterium leprae / genetics; Mycobacterium leprae / metabolism; Plant Proteins / chemistry; Plant Proteins / genetics; Plant Proteins / metabolism; Pyridoxine / biosynthesis; Pyridoxine / genetics; Pyrococcus / genetics; Pyrococcus / metabolism; Sequence Analysis, DNA; Sulfolobus / genetics; Sulfolobus / metabolism; Transformation, Genetic; Triticum / genetics; Triticum / metabolism
TL;DR:
The isolation of a second gene in the newly identified pyridoxine biosynthesis pathway of archaebacteria, some eubacteria, fungi, and plants is described and PDX2 proteins are less well conserved than their PDX1 counterparts but contain several protein motifs that are conserved throughout all PDX 2 proteins.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 patent
Fungal gene encoding resistance to the phytotoxin cercosporin
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2000 patent
Isolated genes and proteins encoding resistance to photosensitizers
Washington, DC: U.S. Patent and Trademark Office.
Source: NC State University Libraries
Added: August 6, 2018
2000 review
The photoactivated Cercospora toxin cercosporin: Contributions to plant disease and fundamental biology
[Review of ]. ANNUAL REVIEW OF PHYTOPATHOLOGY, 38, 461-+.
author keywords: active oxygen; singlet oxygen; photosensitization; polyketide; fungal toxins
TL;DR:
Resistance of Cercospora species to the toxic effects of their own toxin has allowed these organisms to be used as a model for understanding the cellular basis of resistance to singlet oxygen and to general oxidative stress, and the recent discovery that pyridoxine (vitamin B6) quenches singinglet oxygen has led to the understanding of a novel role for this vitamin in cells as well as a novel pathway of biosynthesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2000 journal article
Vitamin B-6 (pyridoxine) and its derivatives are efficient singlet oxygen quenchers and potential fungal antioxidants
PHOTOCHEMISTRY AND PHOTOBIOLOGY, 71(2), 129–134.
TL;DR:
Fluorescence intensity of 3 increased ∼10 times in organic solvents such as ethanol and 1,2-propanediol compared to aqueous solutions, suggesting that fluorescence may be used to image the distribution of 1–4 in Cercospora to understand better the interactions of pyridoxine and 1O2 in the living fungus.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
A highly conserved sequence is a novel gene involved in de novo vitamin B6 biosynthesis
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 96(16), 9374–9378.
author keywords: SOR1; PDX1; photosensitizer; cercosporin
MeSH headings : Animals; Archaea / genetics; Ascomycota / genetics; Ascomycota / growth & development; Ascomycota / metabolism; Aspergillus flavus / genetics; Aspergillus flavus / metabolism; Bacteria / genetics; Bacterial Proteins / genetics; Base Sequence; Biological Evolution; Caenorhabditis elegans / genetics; Conserved Sequence; Databases as Topic; Escherichia coli / genetics; Escherichia coli Proteins; Fungal Proteins / genetics; Ligases; Oxidoreductases; Oxygen; Pyridoxine / biosynthesis; Pyridoxine / genetics; Saccharomyces cerevisiae / genetics; Singlet Oxygen; Transformation, Bacterial
TL;DR:
It is shown that SOR1 is essential in pyridoxine (vitamin B6) synthesis in C. nicotianae and Aspergillus flavus, although it shows no homology to previously identified pyrIDoxine synthesis genes identified in Escherichia coli.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
A novel gene required for cercosporin toxin resistance in the fungus Cercospora nicotianae
Molecular and General Genetics, 262(2), 382–389.
MeSH headings : Amino Acid Sequence; Ascomycota / genetics; Base Sequence; Cloning, Molecular; DNA, Fungal; Fungal Proteins / genetics; Fungal Proteins / physiology; Gene Expression; Genes, Fungal; Genetic Complementation Test; Molecular Sequence Data; Mutation; Perylene / analogs & derivatives; Phenotype; Sequence Homology, Nucleic Acid
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
Source: NC State University Libraries
Added: August 6, 2018
1999 journal article
CFP, the putative cercosporin transporter of Cercospora kikuchii, is required for wild type cercosporin production, resistance, and virulence on soybean
MOLECULAR PLANT-MICROBE INTERACTIONS, 12(10), 901–910.
author keywords: drug/proton antiporter
MeSH headings : Amino Acid Sequence; Ascomycota / genetics; Ascomycota / pathogenicity; Ascomycota / physiology; Base Sequence; Carrier Proteins / genetics; Carrier Proteins / metabolism; DNA, Fungal / genetics; Fungal Proteins / genetics; Fungal Proteins / metabolism; Genetic Complementation Test; Membrane Transport Proteins; Molecular Sequence Data; Open Reading Frames; Perylene / analogs & derivatives; Perylene / metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Soybeans / microbiology; Virulence
TL;DR:
It is proposed that CFP (cercosporin facilitator protein) encodes a cercosporin transporter that contributes resistance to cercOSporin by actively exporting cercsporin, thus maintaining low cellular concentrations of the toxin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1999 journal article
Functional characterization of SOR1, a gene required for resistance to photosensitizing toxins in the fungus Cercospora nicotianae
CURRENT GENETICS, 34(6), 478–485.
author keywords: photosensitizer resistance; singlet oxygen resistance; Cercospora spp.; cercosporin
MeSH headings : Amino Acid Sequence; Ascomycota / drug effects; Ascomycota / genetics; Ascomycota / radiation effects; Base Sequence; Culture Media; DNA Primers / genetics; DNA, Fungal / genetics; Drug Resistance, Microbial / genetics; Fungal Proteins / genetics; Gene Expression Regulation, Fungal / drug effects; Gene Expression Regulation, Fungal / radiation effects; Genes, Fungal; Genetic Complementation Test; Molecular Sequence Data; Mutation; Osmolar Concentration; Perylene / analogs & derivatives; Perylene / pharmacology; Phenotype; Photosensitizing Agents / pharmacology; RNA, Fungal / genetics; RNA, Fungal / metabolism; RNA, Messenger / genetics; RNA, Messenger / metabolism
TL;DR:
Southern analysis indicates that SOR1 is present in other fungi that synthesize structurally related perylenequinone photosensitizers that are complemented toPhotosensitizer resistance by transformation with S OR1.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1998 journal article
Active oxygen in fungal pathogenesis of plants: the role of cercosporin in Cercospora diseases
Recent Advances in Phytochemistry, 32(1998), 31–56.
Source: NC State University Libraries
Added: August 6, 2018
1998 journal article
Roles for mannitol and mannitol dehydrogenase in active oxygen-mediated plant defense
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 95(25), 15129–15133.
TL;DR:
It is shown that the catabolic enzyme mannitol dehydrogenase is induced in a non-mannitol-producing plant in response to both fungal infection and specific inducers of plant defense responses, providing a mechanism whereby the plant can counteract fungal suppression of ROS-mediated defenses by catabolizingMannitol of fungal origin.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(Web of Science)
13. Climate Action
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1998 journal article
SOR1, a gene required for photosensitizer and singlet oxygen resistance in Cercospora fungi, is highly conserved in divergent organisms
MOLECULAR CELL, 1(4), 603–609.
MeSH headings : Cloning, Molecular; Conserved Sequence; DNA, Fungal / analysis; Enzyme Inhibitors / pharmacology; Fungal Proteins / genetics; Fungal Proteins / metabolism; Fungi / drug effects; Fungi / genetics; Gene Expression Regulation, Fungal; Molecular Sequence Data; Mutagenesis / drug effects; Oxygen / metabolism; Perylene / analogs & derivatives; Perylene / pharmacology; Photic Stimulation; Photochemistry; Photosensitizing Agents / pharmacology; Sequence Analysis, DNA; Sequence Homology, Amino Acid
TL;DR:
The characterization of SOR1 provides an additional phenotype to this large group of evolutionarily conserved genes, which is highly conserved in organisms from widely diverse taxa.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1995 journal article
TARGETED GENE DISRUPTION OF CAROTENOID BIOSYNTHESIS IN CERCOSPORA-NICOTIANAE REVEALS NO ROLE FOR CAROTENOIDS IN PHOTOSENSITIZER RESISTANCE
MOLECULAR PLANT-MICROBE INTERACTIONS, 8(4), 569–575.
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1993 journal article
HOST PROTEIN(S) INDUCES ACCUMULATION OF THE TOXIN CERCOSPORIN AND MESSENGER-RNA IN A PHYTOPATHOGENIC STRAIN OF CERCOSPORA-KIKUCHII
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, 43(2), 95–107.
TL;DR:
The data suggest that C. kikuchii responds to soybean protein(s) with increased cercosporin production and changes in transcript accumulation, and this study shows that CM cultures supplemented with aqueous extracts of soybean leaves or meal accumulated between 5 to 65 fold higher levels of cercOSporin than unsupplemented cultures.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
1991 journal article
Isolation of light-enhanced cDNAs of Cercospora kikuchii
Applied and Environmental Microbiology, 57(9), 2671.
Source: NC State University Libraries
Added: August 6, 2018
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