@article{haslauer_moghe_osborne_gupta_loboa_2011, title={Collagen-PCL Sheath-Core Bicomponent Electrospun Scaffolds Increase Osteogenic Differentiation and Calcium Accretion of Human Adipose-Derived Stem Cells}, volume={22}, ISSN={["1568-5624"]}, DOI={10.1163/092050610x521595}, abstractNote={Human adipose-derived stem cells (hASCs) are an abundant cell source capable of osteogenic differentiation, and have been investigated as an autologous stem cell source for bone tissue engineering applications. The objective of this study was to determine if the addition of a type-I collagen sheath to the surface of poly(ε-caprolactone) (PCL) nanofibers would enhance viability, proliferation and osteogenesis of hASCs. This is the first study to examine the differentiation behavior of hASCs on collagen–PCL sheath–core bicomponent nanofiber scaffolds developed using a co-axial electrospinning technique. The use of a sheath–core configuration ensured a uniform coating of collagen on the PCL nanofibers. PCL nanofiber scaffolds prepared using a conventional electrospinning technique served as controls. hASCs were seeded at a density of 20 000 cells/cm2 on 1 cm2 electrospun nanofiber (pure PCL or collagen–PCL sheath–core) sheets. Confocal microscopy and hASC proliferation data confirmed the presence of viable cells after 2 weeks in culture on all scaffolds. Greater cell spreading occurred on bicomponent collagen–PCL scaffolds at earlier time points. hASCs were osteogenically differentiated by addition of soluble osteogenic inductive factors. Calcium quantification indicated cell-mediated calcium accretion was approx. 5-times higher on bicomponent collagen–PCL sheath–core scaffolds compared to PCL controls, indicating collagen–PCL bicomponent scaffolds promoted greater hASC osteogenesis after two weeks of culture in osteogenic medium. This is the first study to examine the effects of collagen–PCL sheath–core composite nanofibers on hASC viability, proliferation and osteogenesis. The sheath–core composite fibers significantly increased calcium accretion of hASCs, indicating that collagen–PCL sheath–core bicomponent structures have potential for bone tissue engineering applications using hASCs.}, number={13}, journal={JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION}, author={Haslauer, Carla Maria and Moghe, Ajit K. and Osborne, Jason A. and Gupta, Bhupender S. and Loboa, Elizabeth G.}, year={2011}, pages={1695–1712} }
 @article{moghe_hufenus_hudson_gupta_2009, title={Effect of the addition of a fugitive salt on electrospinnability of poly(epsilon-caprolactone)}, volume={50}, ISSN={["1873-2291"]}, DOI={10.1016/j.polymer.2009.04.063}, abstractNote={Described in this paper is a novel study focused on producing bead-free ultrafine fibers, with narrow fiber diameter distribution, from Poly(ɛ-caprolactone) (PCL) via electrospinning. High quality product is achieved with the use of a new solvent system that involves an acid–base reaction to produce weak salt complexes, which serve to increase the conductivity of the polymer solution. Additionally, the salt formed dissociates easily and evaporates along with the solvent during the spinning process because its respective acid–base components are volatile at room temperature. This results into the formation of pure PCL nanofibers of ultrafine dimensions. Glacial acetic acid was used as the solvent for the polymer and the organic base pyridine was used to initiate the formation of salt complexes in the solution. Pyridine was added at six different levels to vary the conductivity and examine the latter's effect on fiber morphology. Along with the pyridine content, the polymer concentration was also varied to determine how the two interacted in influencing the size of the fiber and the quality of the structure obtained. It was found that bead-free fibers of sizes lying well within the nano range (140–340 nm) could be produced using the conducting solvent system. Two interesting effects were noted. For a given polymer concentration, the mean fiber diameter increased with increase in pyridine amount. And, lower the polymer concentration, higher was the amount of pyridine required to produce bead-free nanofibers. The combination of these effects along with the fact that the reproducibility of the results was high provided a means of producing fibers with predictable sizes.}, number={14}, journal={POLYMER}, author={Moghe, A. K. and Hufenus, R. and Hudson, S. M. and Gupta, B. S.}, year={2009}, month={Jul}, pages={3311–3318} }
 @article{moghe_gupta_2009, title={Hybrid nanofiber structures for tissue engineering}, volume={9}, number={10}, journal={AATCC Review}, author={Moghe, A. K. and Gupta, B. S.}, year={2009}, pages={43–47} }