@article{ali_reddy_lindgren_jakobek_reddy_2003, title={Differential expression of genes encoding calmodulin-binding proteins in response to bacterial pathogens and inducers of defense responses}, volume={51}, ISSN={["0167-4412"]}, DOI={10.1023/A:1023001403794}, abstractNote={Calmodulin (CaM) plays an important role in sensing and transducing changes in cellular Ca2+ concentration in response to several biotic and abiotic stresses. Although CaM is implicated in plant-pathogen interactions, its molecular targets and their role in defense signaling pathway(s) are poorly understood. To elucidate the signaling pathways that link CaM to defense responses, we screened a cDNA library constructed from bean leaves undergoing a hypersensitive response (HR) with radiolabeled CaM isoforms. A total of 26 putative CBPs were identified. Sequencing of the cDNAs revealed that they represent 8 different genes. They are homologues of previously identified CaM-binding proteins (CBPs) in other systems. However, some CBPs are novel members of known CBP families. The proteins encoded by these clones bound CaM in a Ca2+-dependent manner. To determine if these CBPs are involved in plant defense responses, we analyzed their expression in bean leaves inoculated with compatible, incompatible and nonpathogenic bacterial strains. Expression of three CBPs including an isoform of cyclic nucleotide-gated channels (PvCNGC-A) and two hypothetical proteins (PvCBP60-C and PvCBP60-D) was induced whereas the expression of two other isoforms of CNGCs (PvCNGC-B and PvCNGC-C) was repressed in response to incompatible pathogens. The expression of the rest, a small auxin up RNA (PvSAUR1) and two hypothetical proteins (PvCBP60-A and PvCBP60-B), was not changed. The expression of most of the pathogen-regulated genes was also affected by salicylic acid, jasmonic acid, hydrogen peroxide and a fungal elicitor, which are known to induce defense responses. Our results strongly suggest that at least five bean CBPs are involved in plant defense responses.}, number={6}, journal={PLANT MOLECULAR BIOLOGY}, author={Ali, GS and Reddy, VS and Lindgren, PB and Jakobek, JL and Reddy, ASN}, year={2003}, month={Apr}, pages={803–815} }
 @article{jakobek_lindgren_2002, title={Expression of a bean acid phosphatase cDNA is correlated with disease resistance}, volume={53}, ISSN={["1460-2431"]}, DOI={10.1093/jexbot/53.367.387}, abstractNote={A cDNA clone, designated Hra28 (for hypersensitive reaction associated), was identified corresponding to an RNA transcript that accumulates in bean during the hypersensitive reaction. The Hra28 cDNA is 1084 nucleotides in length and is predicted to encode an acid phosphatase of 264 amino acids. Northern analysis demonstrated that the Hra28 transcript accumulated differentially in response to bacteria which induce a hypersensitive response (HR), a bacterium which causes disease, and a Hrp(-) mutant which does not elicit an HR or cause disease. In contrast, the Hra28 transcript did not accumulate in response to wounding. Thus, the Hra28 gene is induced by multiple stimuli and appears to be regulated in a complex manner.}, number={367}, journal={JOURNAL OF EXPERIMENTAL BOTANY}, author={Jakobek, JL and Lindgren, PB}, year={2002}, month={Feb}, pages={387–389} }
 @article{sullivan_jakobek_lindgren_2001, title={Cloning and characterization of a bean UDP-glucosyltransferase cDNA expressed during plant-bacterial interactions}, volume={14}, ISSN={["0894-0282"]}, DOI={10.1094/MPMI.2001.14.1.90}, abstractNote={ A cDNA clone, which corresponds to an RNA transcript that accumulates in bean during the hypersensitive reaction, was isolated and designated Hra25 (for hypersensitive reaction associated). Hra25 is predicted to encode a UDP-glucosyltransferase. Northern analysis was used to study Hra25 transcript accumulation in bean in response to incompatible and compatible strains of Pseudomonas syringae, an Hrp¯ mutant, and wounding. Our data suggest that the Hra25 transcript is activated in response to specific avr-derived signals as well as non-avr, general signals. }, number={1}, journal={MOLECULAR PLANT-MICROBE INTERACTIONS}, author={Sullivan, TA and Jakobek, JL and Lindgren, PB}, year={2001}, month={Jan}, pages={90–92} }
 @article{jakobek_smith-becker_lindgren_1999, title={A bean cDNA expressed during a hypersensitive reaction encodes a putative calcium-binding protein}, volume={12}, ISSN={["0894-0282"]}, DOI={10.1094/MPMI.1999.12.8.712}, abstractNote={The hypersensitive reaction (HR) is an inducible plant response that is associated with disease resistance. It is characterized by rapid, localized cell death at the site of infection and is believed to inhibit the spread of invading pathogens. We have isolated a cDNA clone, designated Hra32 (for hypersensitive reaction associated), corresponding to an RNA transcript that accumulates in bean during an HR. The predicted protein product of the Hra32 cDNA is an approximately 17 kDa protein of 161 amino acids, with four putative EF-hand calcium-binding domains. The temporal pattern of Hra32 transcript accumulation correlated closely with the onset of the HR in bean after inoculation with incompatible Pseudomonas syringae pv. tabaci and pv. tomato and with tobacco necrosis virus. Hra32 transcript also accumulated in bean in response to compatible P. syringae pv. phaseolicola and was correlated with necrotic cell death associated with disease lesion formation. A more transient pattern of Hra32 transcript accumulation occurred in bean in response to general stimuli that did not result in the HR or host cell death. These treatments included infiltration with a P. syringae pv. tabaci Hrp¯ mutant, P. syringae pv. tabaci cells treated with kanamycin, Escherichia coli, P. fluorescens, or glutathione, and in response to wounding. Thus, there was differential accumulation of the Hra32 transcript in response to specific stimuli resulting in the HR, compared with general stimuli that did not result in cell death. We hypothesize that the Hra32 product may be a component of the pathway that leads to hypersensitive cell death.}, number={8}, journal={MOLECULAR PLANT-MICROBE INTERACTIONS}, author={Jakobek, JL and Smith-Becker, JA and Lindgren, PB}, year={1999}, month={Aug}, pages={712–719} }
 @misc{lindgren_1997, title={The role of hrp genes during plant-bacterial interactions}, volume={35}, ISSN={["0066-4286"]}, DOI={10.1146/annurev.phyto.35.1.129}, abstractNote={▪ Abstract  hrp genes control the ability of phytopathogenic bacteria to cause disease and to elicit hypersensitive reactions on resistant plants. Genetic and biochemical studies have demonstrated that Hrp proteins are components of Type III secretion systems, regulatory proteins, proteinaceous elicitors of the hypersensitive reaction, and enzymes needed for synthesis of periplasmic glucans. Significantly, Type III secretion systems are involved with the secretion of pathogenicity proteins in bacterial pathogens of animals. The transcriptional activation of a number of bacterial avirulence (avr) genes is controlled by Hrp regulatory proteins, and recent experimental evidence suggests that Avr proteins may be transported by Hrp secretion systems. It has also been hypothesized that pathogenicity and/or virulence gene products exit bacterial phytopathogens via Hrp pathways. Thus, hrp genes may be one of the most important groups of genes found in phytopathogenic bacteria in relationship to pathogenicity and host range.}, journal={ANNUAL REVIEW OF PHYTOPATHOLOGY}, author={Lindgren, PB}, year={1997}, pages={129–152} }
 @article{lindgren_jakobek_smith_1992, title={Molecular analysis of plant defense responses to plant pathogens}, volume={24}, number={3}, journal={Journal of Nematology}, author={Lindgren, P. B. and Jakobek, J. L. and Smith, J. A.}, year={1992}, pages={330} }