Trevor Anderson

Works (1)

Updated: July 5th, 2023 15:47

2010 journal article

Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes

BIOTECHNIQUES, 49(5), 807-+.

By: V. DeRocco*, T. Anderson n, J. Piehler *, D. Erie * & K. Weninger n 

author keywords: FRET; MutS; Msh2; Msh6; DNA mismatch repair; SNARE proteins; Munc-18
MeSH headings : Coloring Agents; DNA / chemistry; DNA Mismatch Repair; Energy Transfer; Fluorescence Resonance Energy Transfer / methods; Histidine; Macromolecular Substances / chemistry; MutS DNA Mismatch-Binding Protein / analysis; MutS DNA Mismatch-Binding Protein / chemistry; MutS DNA Mismatch-Binding Protein / pharmacology; Nitrilotriacetic Acid; Nucleic Acid Conformation / drug effects; Protein Conformation / drug effects; SNARE Proteins / chemistry; Tromethamine
TL;DR: These methods are combined to colocalize the mismatch repair protein MutSα on DNA while monitoring MutS α-induced DNA bending using Förster resonance energy transfer (FRET) and to monitor assembly of membrane-tethered SNARE protein complexes. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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