Trevor Anderson
Works (1)
Updated: July 5th, 2023 15:47
2010 journal article
Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes
BIOTECHNIQUES, 49(5), 807-+.
author keywords: FRET; MutS; Msh2; Msh6; DNA mismatch repair; SNARE proteins; Munc-18
MeSH headings : Coloring Agents; DNA / chemistry; DNA Mismatch Repair; Energy Transfer; Fluorescence Resonance Energy Transfer / methods; Histidine; Macromolecular Substances / chemistry; MutS DNA Mismatch-Binding Protein / analysis; MutS DNA Mismatch-Binding Protein / chemistry; MutS DNA Mismatch-Binding Protein / pharmacology; Nitrilotriacetic Acid; Nucleic Acid Conformation / drug effects; Protein Conformation / drug effects; SNARE Proteins / chemistry; Tromethamine
TL;DR:
These methods are combined to colocalize the mismatch repair protein MutSα on DNA while monitoring MutS α-induced DNA bending using Förster resonance energy transfer (FRET) and to monitor assembly of membrane-tethered SNARE protein complexes.
(via Semantic Scholar)
open access via europepmc.org (repository)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
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