@article{gould_estock_hillier_powell_groot_ward_emerson_schal_vickers_2010, title={Sexual isolation of male moths explained by a single pheromone response QTL containing four receptor genes}, volume={107}, ISSN={["0027-8424"]}, DOI={10.1073/pnas.0910945107}, abstractNote={
            Long distance sexual communication in moths has fascinated biologists because of the complex, precise female pheromone signals and the extreme sensitivity of males to specific pheromone molecules. Progress has been made in identifying some genes involved in female pheromone production and in male response. However, we have lacked information on the genetic changes involved in evolutionary diversification of these mate-finding mechanisms that is critical to understanding speciation in moths and other taxa. We used a combined quantitative trait locus (QTL) and candidate gene approach to determine the genetic architecture of sexual isolation in males of two congeneric moths,
            Heliothis subflexa
            and
            Heliothis virescens
            . We report behavioral and neurophysiological evidence that differential male responses to three female-produced chemicals (
            Z
            9-14:Ald,
            Z
            9-16:Ald,
            Z
            11-16:OAc) that maintain sexual isolation of these species are all controlled by a single QTL containing at least four odorant receptor genes. It is not surprising that pheromone receptor differences could control
            H. subflexa
            and
            H. virescens
            responses to
            Z
            9-16:Ald and
            Z
            9-14:Ald, respectively. However, central rather than peripheral level control over the positive and negative responses of
            H. subflexa
            and
            H. virescens
            to
            Z
            11-16:OAc had been expected. Tight linkage of these receptor genes indicates that mutations altering male response to complex blends could be maintained in linkage disequilibrium and could affect the speciation process. Other candidate genes such as those coding for pheromone binding proteins did not map to this QTL, but there was some genetic evidence of a QTL for response to
            Z
            11-16:OH associated with a sensory neuron membrane protein gene.
          }, number={19}, journal={PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA}, author={Gould, Fred and Estock, Marie and Hillier, N. Kirk and Powell, Bekah and Groot, Astrid T. and Ward, Catherine M. and Emerson, Jennifer L. and Schal, Coby and Vickers, Neil J.}, year={2010}, month={May}, pages={8660–8665} }