@article{ashwell_ceddia_house_cassady_eisen_eling_collins_grissom_odle_2010, title={Trans-10, cis-12-conjugated linoleic acid alters hepatic gene expression in a polygenic obese line of mice displaying hepatic lipidosis}, volume={21}, ISSN={["1873-4847"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-77955844665&partnerID=MN8TOARS}, DOI={10.1016/j.jnutbio.2009.06.013}, abstractNote={The trans-10, cis-12 isomer of conjugated linoleic acid (CLA) causes a rapid reduction of body and adipose mass in mice. In addition to changes in adipose tissue, numerous studies have reported alterations in hepatic lipid metabolism. Livers of CLA-fed mice gain mass, partly due to lipid accumulation; however, the precise molecular mechanisms are unknown. To elucidate these mechanisms, we examined fatty acid composition and gene expression profiles of livers from a polygenic obese line of mice fed 1% trans-10, cis-12-CLA for 14 days. Analysis of gene expression data led to the identification of 1393 genes differentially expressed in the liver of CLA-fed male mice at a nominal P value of .01, and 775 were considered significant using a false discovery rate (FDR) threshold of .05. While surprisingly few genes in lipid metabolism were impacted, pathway analysis found that protein kinase A (PKA) and cyclic adenosine monophosphate (cAMP) pathways signaling pathways were affected by CLA treatment and 98 of the 775 genes were found to be regulated by hepatocyte nuclear factor 4alpha, a transcription factor important in controlling liver metabolic status.}, number={9}, journal={JOURNAL OF NUTRITIONAL BIOCHEMISTRY}, author={Ashwell, Melissa S. and Ceddia, Ryan P. and House, Ralph L. and Cassady, Joseph P. and Eisen, Eugene J. and Eling, Thomas E. and Collins, Jennifer B. and Grissom, Sherry F. and Odle, Jack}, year={2010}, month={Sep}, pages={848–855} }