Nimish Gera

Works (7)

Updated: July 7th, 2023 21:18

2013 journal article

Generation and Properties of Antibacterial Coatings Based on Electrostatic Attachment of Silver Nanoparticles to Protein-Coated Polypropylene Fibers

ACS APPLIED MATERIALS & INTERFACES, 5(11), 5298–5306.

By: K. Goli n, N. Gera n, X. Liu n, B. Rao n , O. Rojas n & J. Genzer n 

Contributors: K. Goli n, N. Gera n, X. Liu n, B. Rao n , O. Rojas n & J. Genzer n 

author keywords: antibacterial; polypropylene; protein adsorption; nanoparticle; functional coating
MeSH headings : Anti-Bacterial Agents / chemistry; Coated Materials, Biocompatible / chemistry; Escherichia coli / drug effects; Escherichia coli Infections / prevention & control; Hydrophobic and Hydrophilic Interactions; Light; Metal Nanoparticles / chemistry; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Polypropylenes / chemistry; Proteins / chemistry; Silver / chemistry; Static Electricity; Ultraviolet Rays
TL;DR: The results show that PP surfaces coated with AgNPs exhibit excellent antibacterial activity with 100% removal efficiency and are tested against gram negative bacteria Escherichia coli. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2013 journal article

Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast

ACS APPLIED MATERIALS & INTERFACES, 5(19), 9349–9354.

By: M. Canbolat  n, N. Gera n, C. Tang n, B. Monian n, B. Rao n , B. Pourdeyhimi n , S. Khan n 

Contributors: M. Canbolat  n, N. Gera n, C. Tang n, B. Monian n, B. Rao n , B. Pourdeyhimi n , S. Khan n 

author keywords: electrospinning; nanofiber; protein; biocatalyst immobilization; yeast surface display
MeSH headings : Biocatalysis; Cell Survival / drug effects; Green Fluorescent Proteins; Microscopy, Electron, Scanning; Nanofibers / chemistry; Polymers / chemistry; Polymers / pharmacology; Polyvinyl Alcohol / chemistry; Polyvinyl Alcohol / pharmacology; Protein Conformation / drug effects; Saccharomyces cerevisiae / chemistry; Saccharomyces cerevisiae / drug effects; Saccharomyces cerevisiae / growth & development; Water / chemistry
TL;DR: This method of processing functionalized yeast may be a powerful tool in the direct immobilization of properly folded, active enzymes within electrospun nanofibers with potential applications in biocatalysis. (via Semantic Scholar)
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Sources: Web Of Science, NC State University Libraries, ORCID
Added: August 6, 2018

2012 journal article

Avidity-mediated virus separation using a hyperthermophilic affinity ligand

BIOTECHNOLOGY PROGRESS, 29(1), 237–246.

By: M. Hussain n, D. Lockney n, R. Wang n, N. Gera n & B. Rao n 

Contributors: M. Hussain n, D. Lockney n, R. Wang n, N. Gera n & B. Rao n 

author keywords: avidity; affinity ligand; hyperthermophilic; multivalent
MeSH headings : Archaeal Proteins / chemistry; Archaeal Proteins / isolation & purification; Archaeal Proteins / metabolism; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / isolation & purification; DNA-Binding Proteins / metabolism; Ligands; Molecular Weight; Plant Extracts; Plant Leaves / virology; Sulfolobus solfataricus / chemistry; Sulfolobus solfataricus / metabolism; Tobacco / virology; Tombusviridae / isolation & purification
TL;DR: The approach may be broadly relevant to cases where capture of large multivalent species from complex mixtures and subsequent release without the use of harsh elution conditions is necessary, and bypasses chemical conjugation of the affinity ligand to the capture surface. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold

PLOS ONE, 7(11).

By: N. Gera n, A. Hill n, D. White  n, R. Carbonell n & B. Rao n 

Contributors: N. Gera n, A. Hill n, D. White  n, R. Carbonell n & B. Rao n 

MeSH headings : Carrier Proteins / genetics; Carrier Proteins / metabolism; Epitopes / genetics; Escherichia coli / genetics; Escherichia coli / metabolism; Histidine / genetics; Histidine / metabolism; Humans; Hydrogen-Ion Concentration; Immunoglobulin Fc Fragments / genetics; Immunoglobulin Fc Fragments / metabolism; Immunoglobulin G / genetics; Immunoglobulin G / metabolism; Mutagenesis; Sulfolobus solfataricus / genetics; Sulfolobus solfataricus / metabolism
TL;DR: P pH sensitive hFc binders are attractive candidates for use in chromatography, to achieve elution of IgG under milder pH conditions, but further studies are needed to evaluate if the Sso7d mutants identified in this study are indeed useful as affinity ligands in Chromatography. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 review

Protein selection using yeast surface display

[Review of ]. METHODS, 60(1), 15–26.

By: N. Gera n, M. Hussain n & B. Rao n 

Contributors: N. Gera n, M. Hussain n & B. Rao n 

author keywords: Yeast surface display; Protein engineering; Library screening; Biophysical characterization; Alternative scaffolds
MeSH headings : Combinatorial Chemistry Techniques; Flow Cytometry; Protein Engineering / methods; Yeasts / genetics; Yeasts / metabolism
TL;DR: This review presents detailed protocols for the use of yeast surface display to isolate de novo binding proteins from combinatorial libraries, and subsequent biophysical characterization of binders. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2012 journal article

Scaffold Diversification Enhances Effectiveness of a Super library of Hyperthermophilic Proteins

ACS SYNTHETIC BIOLOGY, 2(1), 6–13.

By: M. Hussain n, N. Gera n, A. Hill n & B. Rao n 

Contributors: M. Hussain n, N. Gera n, A. Hill n & B. Rao n 

author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; alternate scaffold; mRNA display
MeSH headings : Biotechnology / methods; Carrier Proteins / genetics; Carrier Proteins / metabolism; Fungal Proteins / chemistry; Fungal Proteins / genetics; Fungal Proteins / metabolism; Models, Molecular; Peptide Library; Protein Conformation; Yeasts / genetics; Yeasts / metabolism
TL;DR: Scaffold diversification, i.e., randomization of multiple different scaffolds, is a powerful alternate strategy for combinatorial library construction and binders from this modestly sized superlibrary have affinities comparable or higher than those obtained from a library with 1000-fold higher sequence diversity but derived from a single stable scaffold. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

2011 journal article

Highly Stable Binding Proteins Derived from the Hyperthermophilic Sso7d Scaffold

JOURNAL OF MOLECULAR BIOLOGY, 409(4), 601–616.

By: N. Gera n, M. Hussain n, R. Wright  n & B. Rao n 

Contributors: N. Gera n, M. Hussain n, R. Wright  n & B. Rao n 

author keywords: hyperthermophilic protein scaffolds; yeast surface display; protein engineering; stability; alternate scaffold
MeSH headings : Amino Acid Sequence; Animals; Archaeal Proteins / chemistry; Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Chickens; DNA Mutational Analysis; DNA-Binding Proteins / chemistry; DNA-Binding Proteins / genetics; DNA-Binding Proteins / metabolism; Fluorescein / chemistry; Fluorescein / metabolism; Immunoglobulins / chemistry; Immunoglobulins / metabolism; Mice; Molecular Sequence Data; Muramidase / chemistry; Muramidase / metabolism; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Protein Denaturation; Streptavidin / chemistry; Streptavidin / metabolism; Sulfolobus solfataricus / chemistry; beta Catenin / chemistry; beta Catenin / metabolism
TL;DR: It is shown that highly stable binding proteins for a wide spectrum of targets can be generated through mutagenesis of the Sso7d protein from the hyperthermophilic archaeon Sulfolobus solfataricus, and that S so7d-derived binding proteins could discriminate between closely related immunoglobulins. (via Semantic Scholar)
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Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018

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