@article{bai_qu_2001, title={Factors influencing tissue culture responses of mature seeds and immature embryos in turf-type tall fescue}, volume={120}, ISSN={["0179-9541"]}, DOI={10.1046/j.1439-0523.2001.00594.x}, abstractNote={AbstractIn an effort to optimize tissue culture responses of turf‐type tall fescue for genetic transformation, the effects of five culture medium supplements on tissue culture responses were investigated with immature embryos and mature seeds of an elite cultivar, ‘Coronado’, as explant tissues. For both explants, calli induced on 6‐benzylaminopurine (BAP)‐containing medium had significantly improved regeneration ability. The optimal concentration of BAP for the induction of regenerable callus from mature seeds was 0.1 mg/l. Thidiazuron also improved callus regeneration frequency of mature seeds. Casein hydrolysate, L‐proline and myo‐inositol improved callus induction of immature embryos but not mature seeds. They did not improve callus regeneration frequencies with either explant tissue. By simply slicing the mature seeds into two halves longitudinally, the callus induction frequencies, as well as the corresponding overall plant regeneration frequencies, were increased approximately three‐ to six‐fold in all three combinations of 2,4‐dichlorophenoxyacetic acid and BAP in callus induction media.}, number={3}, journal={PLANT BREEDING}, author={Bai, Y and Qu, R}, year={2001}, month={Jun}, pages={239–242} } @article{bai_qu_2000, title={An evaluation of callus induction and plant regeneration in twenty-five turf-type tall fescue (Festuca arundinacea Schreb.) cultivars}, volume={55}, ISSN={["0142-5242"]}, DOI={10.1046/j.1365-2494.2000.00235.x}, abstractNote={In order to investigate the genetic variation in tissue culture response and to find the cultivars with high regeneration ability for genetic transformation, twenty‐five turf‐type tall fescue (Festuca arundinacea Schreb.) cultivars, including many elite ones released recently, were evaluated for their callus induction and plant regeneration responses. Callus induction was initiated from mature seeds on a Murashige and Skoog (MS) medium containing 9·0 mg l–1 2,4‐dichlorophenoxyacetic acid (2,4‐D). Induced calli were subcultured on the same medium with 2·0 mg l–1 2,4‐D and then transferred to a MS medium supplemented with 2·5 mg l–1 6‐benzylaminopurine (BAP) for plant regeneration. Significant differences were observed among the twenty‐five cultivars in both callus induction and plant regeneration (P < 0·001). Callus induction rate of viable seeds varied from 4·4% to 51·9%. Callus regeneration rates ranged from 16·7% to 58·8%. Overall regeneration rates (number of regenerated calli over number of cultured viable seeds) ranged from 1% to 22%. Approximately 94% of the regenerants were green plantlets.}, number={4}, journal={GRASS AND FORAGE SCIENCE}, author={Bai, Y and Qu, R}, year={2000}, month={Dec}, pages={326–330} } @article{bai_kelly_1999, title={A study of photosynthetic activities of eight asparagus genotypes under field conditions}, volume={124}, number={1}, journal={Journal of the American Society for Horticultural Science}, author={Bai, Y. Y. and Kelly, J. F.}, year={1999}, pages={61–66} }