2011 journal article

A photocrosslinking assay for reporting protein interactions in polyketide and fatty acid synthases

Molecular BioSystems, 7(11), 3152.

By: Z. Ye n, M. Bair n, H. Desai n & G. Williams n

MeSH headings : Acyl Carrier Protein / chemistry; Acyl Carrier Protein / metabolism; Codon, Terminator; Cross-Linking Reagents / chemistry; Cross-Linking Reagents / metabolism; Fatty Acid Synthases / chemistry; Fatty Acid Synthases / metabolism; Kinetics; Mutagenesis, Site-Directed; Polyketide Synthases / chemistry; Polyketide Synthases / metabolism; Polyketides / metabolism; Protein Conformation
TL;DR: This work reports a facile strategy for rapidly reporting such ACP-KS interactions based on the incorporation of an amino acid with photocrosslinking functionality that can be applied to any polyketide or fatty acid synthase regardless of substrate specificity. (via Semantic Scholar)
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Added: August 6, 2018

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