@article{dhammi_krestchmar_ponnusamy_bacheler_reisig_herbert_del pozo-valdivia_roe_2016, title={Biology, pest status, microbiome and control of kudzu bug (Hemiptera: Heteroptera: Plataspidae): a new invasive pest in the U.S.}, volume={17}, ISSN={["1422-0067"]}, url={http://dx.doi.org/10.3390/ijms17091570}, DOI={10.3390/ijms17091570}, abstractNote={Soybean is an important food crop, and insect integrated pest management (IPM) is critical to the sustainability of this production system. In recent years, the introduction into the United States of the kudzu bug currently identified as Megacopta cribraria (F.), poses a threat to soybean production. The kudzu bug was first discovered in the state of Georgia, U.S. in 2009 and since then has spread to most of the southeastern states. Because it was not found in the North American subcontinent before this time, much of our knowledge of this insect comes from research done in its native habitat. However, since the U.S. introduction, studies have been undertaken to improve our understanding of the kudzu bug basic biology, microbiome, migration patterns, host selection and management in its expanding new range. Researchers are not only looking at developing IPM strategies for the kudzu bug in soybean, but also at its unique relationship with symbiotic bacteria. Adult females deposit bacterial packets with their eggs, and the neonates feed on these packets to acquire the bacteria, Candidatus Ishikawaella capsulata. The kudzu bug should be an informative model to study the co-evolution of insect function and behavior with that of a single bacteria species. We review kudzu bug trapping and survey methods, the development of bioassays for insecticide susceptibility, insecticide efficacy, host preferences, impact of the pest on urban environments, population expansion, and the occurrence of natural enemies. The identity of the kudzu bug in the U.S. is not clear. We propose that the kudzu bug currently accepted as M. cribraria in the U.S. is actually Megacopta punctatissima, with more work needed to confirm this hypothesis.}, number={9}, journal={Int. J. Mol. Sci}, publisher={MDPI AG}, author={Dhammi, Anirudh and Krestchmar, Jaap B. and Ponnusamy, Loganathan and Bacheler, Jack S. and Reisig, Dominic D. and Herbert, Ames and Del Pozo-Valdivia, Alejandro I. and Roe, R. Michael}, year={2016} } @article{magalhaes_kretschmar_donohue_roe_2013, title={Pyrosequencing of the adult tarnished plant bug, Lygus lineolaris, and characterization of messages important in metabolism and development}, volume={146}, ISSN={["1570-7458"]}, DOI={10.1111/eea.12035}, abstractNote={AbstractThe adoption of Bt transgenic cotton has practically eliminated lepidopteran pests from this crop and has produced a secondary pest problem, with pierce‐sucking insects such as the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae). The future of cotton genetic pest management is threatened by these insects and their development of resistance to chemical insecticides. Lygus lineolaris is also a pest of more than 100 other crops. The development of transcriptome data for this insect should be transformative in essentially all aspects of research on plant bug biology and the development of control strategies. The first 454 tarnished plant bug whole body (WB) and gut (G) transcriptomes were constructed (half plate for each). A total of 116 163 527 bases were obtained, representing 262 555 WB and 229 919 G reads (SRA048217) of which 232 058 (SRS280903) and 168 069 (SRS280894) reads, respectively, were available for assembly. The average read length was 233.1 and 208.5 bp for WB and G, respectively. The whole body and gut reads were assembled together (WB‐G) to produce the most complete transcriptome possible from our sequencing effort and resulted in 6 970 contigs with an average length of 393 bp. The gut transcriptome alone was assembled into 3 549 contigs with an average length of 349 bp. The smallest contig was 55 bp and the largest was 3 466 bp, and there were 62 484 sequences that could not be assembled (singletons) among both transcriptomes. Overall transcriptome analysis was organized according to the Gene Ontology consortium, enzyme commission, and InerPro using the Blast2GO® program. We further characterized metabolic systems and messages associated with development.}, number={3}, journal={ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA}, author={Magalhaes, Leonardo C. and Kretschmar, Jaap B. and Donohue, Kevin V. and Roe, R. Michael}, year={2013}, month={Mar}, pages={364–378} } @article{kretschmar_cabrera_bradley_roe_2013, title={Novel adult feeding disruption test (FDT) to detect insecticide resistance of lepidopteran pests in cotton}, volume={69}, ISSN={["1526-4998"]}, DOI={10.1002/ps.3420}, abstractNote={AbstractBACKGROUNDResistance monitoring is an important aspect of insect resistance management and the preservation of insecticide efficacy. The adult vial test (AVT) is most often used for resistance monitoring for a variety of insects. A potential alternative method is feeding disruption where resistant insects are distinguished from susceptible insects on the basis of their ability to feed on insecticide in nectar containing a colorimetric marker to measure feeding. The advantages of a feeding disruption test (FDT) for lepidopteran adults might include a more rapid assay than AVT, an assay format easier to prepare, a bioassay applicable to both oral and contact insecticides and the provision of food and water during the course of the test. The objective of the present work was to determine the feasibility of an adult FDT.RESULTSHeliothis virescens moths fed permethrin and spinosad in dyed nectar yielded dose‐dependent ingestion, fecal production and mortality data. A permethrin diagnostic dose distinguished pyrethroid‐resistant from pyrethroid‐susceptible moths, based on fecal production.CONCLUSIONProof of concept was demonstrated for an adult FDT in which resistant moths were distinguished from susceptible moths on the basis of the ability of the insect to feed on insecticide in dyed nectar and produce dyed feces.© 2012 Society of Chemical Industry}, number={5}, journal={PEST MANAGEMENT SCIENCE}, author={Kretschmar, B. and Cabrera, Ana R. and Bradley, Julius R. and Roe, R. Michael}, year={2013}, month={May}, pages={652–660} } @article{magalhaes_van kretschmar_barlow_roe_walgenbach_2012, title={Development of a rapid resistance monitoring bioassay for codling moth larvae}, volume={68}, ISSN={["1526-4998"]}, DOI={10.1002/ps.3246}, abstractNote={AbstractBACKGROUND: The codling moth, Cydia pomonella (L.), is one of the most important pests of apple worldwide. Use of insecticides for management of this insect has been extensive and has resulted in resistance development. There are a number of different bioassay methods to monitor for codling moth resistance; however, many are not applicable to new insecticides and most are time consuming. A novel 16‐well plasticware bioassay plate containing lyophilized diet was developed for rapid resistance monitoring of codling moth.RESULTS: The contact insecticides acetamiprid and azinphosmethyl were significantly more toxic to neonates than to fourth instars. However, there was no significant difference in LC50 values between neonates and fourth instars to the ingestion insecticides chlorantraniliprole, methoxyfenozide, novaluron and spinetoram. Field colonies of codling moth were significantly more resistant to methoxyfenozide than susceptible populations. A diagnostic dose of 20 µg mL−1 (LC99) was established to monitor for codling moth resistance to methoxyfenozide.CONCLUSIONS: The results presented here demonstrate that a novel and rapid bioassay can be used to monitor for codling moth resistance to methoxyfenozide. The bioassay method is relevant to both ingestion and contact insecticides, but a single diagnostic dose, regardless of larval age, is only relevant to ingestion insecticides. Age‐dependent diagnostic doses are likely necessary for contact insecticides. Copyright © 2011 Society of Chemical Industry}, number={6}, journal={PEST MANAGEMENT SCIENCE}, author={Magalhaes, Leonardo C. and Van Kretschmar, Jaap B. and Barlow, Vonny M. and Roe, R. Michael and Walgenbach, James F.}, year={2012}, month={Jun}, pages={883–888} }