Joseph E. Alexander

Works (11)

Updated: July 5th, 2023 16:02

2015 journal article

Maternal serum progesterone concentration and early conceptus development of bovine embryos produced in vivo or in vitro

DOMESTIC ANIMAL ENDOCRINOLOGY, 52, 75–81.

By: C. Barnwell n, P. Farin n, C. Whisnant n, J. Alexander n & C. Farin n

author keywords: Cattle; Embryo; Conceptus; Progesterone; In vitro production; Embryo transfer
MeSH headings : Animals; Blastocyst / physiology; Cattle / embryology; Embryo Transfer / veterinary; Embryonic Development / physiology; Female; Fertilization in Vitro / veterinary; Gestational Age; Male; Pregnancy; Progesterone / blood; Superovulation
TL;DR: Differences in autonomy may exist between in vitro- and in vivo-produced embryos during the period of conceptus elongation with in vitro/in vivo- produced embryos relying more on intrinsic factors to influence elongation. (via Semantic Scholar)
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Added: August 6, 2018

2010 journal article

Expression of messenger RNAs for insulin-like growth factors and their receptors in bovine fetuses at early gestation from embryos produced in vivo or in vitro

THERIOGENOLOGY, 74(7), 1288–1295.

By: C. Farin n, J. Alexander n & P. Farin n

author keywords: Embryo; In vitro production; Abnormal Offspring Syndrome; Gene expression; IGF
MeSH headings : Animals; Cattle; Female; Fertilization in Vitro / veterinary; Fetus / metabolism; Gene Expression Regulation, Developmental / physiology; Liver / metabolism; Male; Muscle, Skeletal / metabolism; Pregnancy; RNA, Messenger / genetics; RNA, Messenger / metabolism; Receptor, IGF Type 1 / genetics; Receptor, IGF Type 1 / metabolism; Receptor, IGF Type 2 / genetics; Receptor, IGF Type 2 / metabolism; Somatomedins / genetics; Somatomedins / metabolism
TL;DR: In conclusion, in vitro embryo culture was associated with subtle changes in fetal development as well as altered expression of both imprinted and non-imprinted genes. (via Semantic Scholar)
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Added: August 6, 2018

2007 journal article

The role of transcription in EGF- and FSH-mediated oocyte maturation in vitro

ANIMAL REPRODUCTION SCIENCE, 98(1-2), 97–112.

By: C. Farin n, K. Rodriguez*, J. Alexander n, J. Hockney n, J. Herrick * & S. Kennedy-Stoskopf n

author keywords: meiosis; FSH; EGF; oocyte maturation; gene transcription
MeSH headings : Animals; Cell Culture Techniques; Dichlororibofuranosylbenzimidazole / pharmacology; Epidermal Growth Factor / pharmacology; Female; Follicle Stimulating Hormone / pharmacology; Horses; Mammals; Mice; Mitosis; Oocytes / drug effects; Oocytes / physiology; Transcription, Genetic
TL;DR: Results presented support the conclusions that EGF-induced oocytes maturation does not require nascent gene transcription in both mice and domestic cats and FSH requires gene transcription to induce oocyte maturation in both species. (via Semantic Scholar)
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2006 journal article

Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression

PHYSIOLOGICAL GENOMICS, 27(3), 318–327.

By: K. Rodriguez n, L. Blomberg*, K. Zuelke*, J. Miles  n, J. Alexander n & C. Farin n

MeSH headings : Animals; Cell Culture Techniques; Cell Separation; Dichlororibofuranosylbenzimidazole / pharmacology; Female; Follicle Stimulating Hormone / metabolism; Gene Expression Profiling / methods; Meiosis / drug effects; Meiosis / genetics; Mice; Oocytes / drug effects; Oocytes / physiology; Ovary / cytology; RNA, Messenger / genetics; RNA, Messenger / metabolism; Transcription, Genetic / drug effects
TL;DR: The critical period of transcription required for GVBD occurred between 15 and 30 min after culture initiation, and 39 and 27 transcripts were identified as differentially expressed at the P < or = 0.01 and P </= 0.001 levels. (via Semantic Scholar)
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2005 journal article

Effects of embryo culture on angiogenesis and morphometry of bovine placentas during early gestation

BIOLOGY OF REPRODUCTION, 73(4), 663–671.

By: . Miles  n, C. Farin n, K. Rodriguez n, J. Alexander n & P. Farin n

author keywords: developmental biology; embryo; gene regulation; in vitro fertilization; placenta
MeSH headings : Animals; Blastocyst / physiology; Blood Vessels / anatomy & histology; Cattle; Coculture Techniques; Culture Media / pharmacology; Culture Media, Serum-Free / pharmacology; Embryo Culture Techniques; Embryo, Mammalian / anatomy & histology; Female; Fertilization in Vitro / methods; Gene Expression; Gene Expression Regulation, Developmental; Gestational Age; Neovascularization, Physiologic; PPAR gamma / genetics; PPAR gamma / metabolism; Placenta / anatomy & histology; Placenta / blood supply; Placenta / drug effects; Pregnancy; Vascular Endothelial Growth Factor A / genetics; Vascular Endothelial Growth Factor A / metabolism
TL;DR: Placentas from embryos produced in vitro using a semidefined culture system exhibited a greater degree of aberrant development of the placenta during early gestation than with placentas produced in vivo or in vivo using an undefined culture system. (via Semantic Scholar)
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2004 journal article

Angiogenesis and morphometry of bovine placentas in late gestation from embryos produced in vivo or in vitro

BIOLOGY OF REPRODUCTION, 71(6), 1919–1926.

By: . Miles  n, C. Farin n, K. Rodriguez n, J. Alexander n & P. Farin n

author keywords: developmental biology; embryo; gene regulation; in vitro fertilization; placenta
MeSH headings : Animals; Blood Vessels / anatomy & histology; Cattle; Chorionic Villi / anatomy & histology; Female; Fetal Weight; Gestational Age; Neovascularization, Physiologic; PPAR gamma / genetics; PPAR gamma / metabolism; Placenta / anatomy & histology; Placenta / blood supply; Pregnancy; RNA, Messenger / metabolism; Vascular Endothelial Growth Factor A / genetics; Vascular Endothelial Growth Factor A / metabolism
TL;DR: Findings are consistent with the concept that compensatory mechanisms exist in the vascular beds of placentas from bovine embryos produced in vitro. (via Semantic Scholar)
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2002 journal article

Development of skeletal muscle and expression of candidate genes in bovine fetuses from embryos produced in vivo or in vitro

BIOLOGY OF REPRODUCTION, 67(2), 401–408.

By: A. Crosier n, C. Farin n, K. Rodriguez n, P. Blondin n, J. Alexander n & P. Farin n

author keywords: conceptus; developmental biology; embryo; fetus; gene regulation; in vitro fertilization
MeSH headings : Animals; Blastocyst / physiology; Body Weight / physiology; Cattle; DNA Primers; Electrophoresis, Agar Gel; Embryo Transfer; Embryonic and Fetal Development / physiology; Female; Fertilization in Vitro; Fetus / metabolism; Gene Expression Regulation, Developmental / genetics; Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism; In Vitro Techniques; Indicators and Reagents; Male; Muscle Fibers, Skeletal / physiology; Muscle Proteins / biosynthesis; Muscle Proteins / genetics; Muscle, Skeletal / anatomy & histology; Muscle, Skeletal / embryology; Pregnancy; RNA / biosynthesis; RNA / genetics; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: In vitro production of embryos resulted in fetuses with altered development of skeletal muscle fibers, and myostatin was identified as the candidate gene whose expression may contribute to the observed changes in muscle development of these fetuses. (via Semantic Scholar)
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2001 journal article

Ultrastructural morphometry of bovine blastocysts produced in vivo or in vitro

BIOLOGY OF REPRODUCTION, 64(5), 1375–1385.

By: A. Crosier n, P. Farin n, M. Dykstra n, J. Alexander n & C. Farin n

author keywords: early development; IVF/ART
MeSH headings : Animals; Apoptosis; Blastocyst / ultrastructure; Blastomeres / ultrastructure; Cattle / embryology; Cell Nucleus / ultrastructure; Culture Media; Culture Techniques; Cytoplasm / ultrastructure; Fertilization in Vitro / veterinary; Microscopy, Electron; Mitochondria / ultrastructure; Superovulation; Vacuoles / ultrastructure
TL;DR: Blastocysts produced in vitro possessed deviations in volume densities of organelles associated with cellular metabolism as well as deviations associated with altered embryonic differentiation, however, the specific nature of these deviations varied with the type of culture conditions used for in vitro embryo production. (via Semantic Scholar)
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2000 journal article

In vitro production of embryos alters levels of insulin-like growth factor-II messenger ribonucleic acid in bovine fetuses 63 days after transfer

BIOLOGY OF REPRODUCTION, 62(2), 384–389.

By: P. Blondin n, P. Farin n, A. Crosier n, J. Alexander n & C. Farin n

MeSH headings : Animals; Cattle; Embryo Transfer; Embryo, Mammalian / metabolism; Female; Fertilization in Vitro; Fetus / metabolism; Gestational Age; In Vitro Techniques; Insulin-Like Growth Factor II / biosynthesis; Liver / metabolism; Muscle, Skeletal / metabolism; Pregnancy; RNA, Messenger / biosynthesis; RNA, Ribosomal / biosynthesis; Ribonucleases / analysis
TL;DR: Fetuses originating from in vitro production systems possessed altered levels of IGF-II mRNA in both liver and skeletal muscle at Day 70 of gestation, according to the objective of this study. (via Semantic Scholar)
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2000 journal article

Ultrastructural morphometry of bovine compact morulae produced in vivo or in vitro

BIOLOGY OF REPRODUCTION, 62(5), 1459–1465.

By: A. Crosier n, P. Farin n, M. Dykstra n, J. Alexander n & C. Farin n

MeSH headings : Animals; Cattle; Cell Culture Techniques / methods; Cytoplasm; Female; Fertilization in Vitro; Morula / cytology; Morula / ultrastructure
TL;DR: Compound morulae produced in IVPS culture medium possessed the greatest deviations in cellular ultrastructure, compared with those produced in vivo. (via Semantic Scholar)
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1999 journal article

Does in vitro culture affect the expression of insulin-like growth factor-II (IGF-II) messenger RNA in fetal bovine liver?

Biology of Reproduction, 60(1999), 248–249.

By: P. Blondin, P. Farin, A. Crosier, J. Alexander & C. Farin

Source: NC State University Libraries
Added: August 6, 2018

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