@article{mankin_allen_phelan_spiker_thompson_2003, title={Elevation of transgene expression level by flanking matrix attachment regions (MAR) is promoter dependent: a study of the interactions of six promoters with the RB7 3 ' MAR}, volume={12}, ISSN={["0962-8819"]}, DOI={10.1023/A:1022194120518}, abstractNote={We have analyzed effects of a matrix attachment region (MAR) from the tobacco RB7 gene on transgene expression from six different promoters in stably transformed tobacco cell cultures. The presence of MARs flanking the transgene increased expression of constructs based on the constitutive CaMV 35S, NOS, and OCS promoters. Expression from an induced heat shock promoter was also increased and MARs did not cause expression in the absence of heat shock. There was also no effect of MARs on the pea ferredoxin promoter, which is not normally expressed in this cell line. Importantly, most transgenes flanked by RB7 MAR elements showed a large reduction in the number of low expressing GUS transformants relative to control constructs without MARs.}, number={1}, journal={TRANSGENIC RESEARCH}, author={Mankin, SL and Allen, GC and Phelan, T and Spiker, S and Thompson, WF}, year={2003}, month={Feb}, pages={3–12} } @article{mankin_thompson_2001, title={New green fluorescent protein genes for plant transformation: Intron-containing, ER-localized, and soluble-modified}, volume={19}, DOI={10.1007/bf02824074}, number={1}, journal={Plant Molecular Biology Reporter}, author={Mankin, S. L. and Thompson, William}, year={2001}, pages={13–26} } @misc{thompson_allen_mankin_2000, title={Increasing expression of transgenes in plant cells using insulator elements}, volume={6,100,448}, number={2000 Aug. 8}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Thompson, W. and Allen, G. and Mankin, S.}, year={2000} } @misc{thompson_allen_mankin_2000, title={Method for reducing expression variability of transgenes in plant cells}, volume={6,037,525}, number={2000 Mar. 14}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Thompson, W. and Allen, G. and Mankin, S.}, year={2000} } @article{mankin_allen_thompson_1997, title={Introduction of a plant intron into the luciferase gene of Photinus pyralis}, volume={15}, DOI={10.1007/bf02812270}, number={2}, journal={Plant Molecular Biology Reporter}, author={Mankin, S. L. and Allen, G. C and Thompson, William}, year={1997}, pages={186–196} }