@misc{kullen_klaenhammer_2001, title={Acid-inducible promoters for gene expression}, volume={6,242,194}, number={2001 June 5}, publisher={Washington, DC: U.S. Patent and Trademark Office}, author={Kullen, M. J. and Klaenhammer, T. R.}, year={2001} } @article{kullen_klaenhammer_2000, title={Genetic modification of intestinal lactobacilli and bifidobacteria}, volume={2}, ISBN={1467-3037}, DOI={10.21775/cimb.002.041}, abstractNote={Lactobacilli and bifidobacteria are important members of the gastrointestinal microflora of man and animals. There is a substantial and growing body of evidence that these microbes provide benefits to the host in which they reside. Understanding the roles of these two groups of bacteria in the intestine continues to be a significant challenge. To this end, genetic characterisation and manipulation of intestinal lactobacilli and bifidobacteria is essential to define their contributions to the intestinal microflora, and to potentially exploit any beneficial or unique properties. This review will describe the tools and strategies currently available for the genetic manipulation of lactobacilli and bifidobacteria. Additionally, the ramifications and opportunities that may arise as a result of the genetic manipulation of probiotic lactobacilli and bifidobacteria will be addressed.}, number={2}, journal={Current Issues in Molecular Biology}, author={Kullen, M. J. and Klaenhammer, T. R.}, year={2000}, pages={41} } @article{kullen_sanozky-dawes_crowell_klaenhammer_2000, title={Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex}, volume={89}, ISSN={["1364-5072"]}, DOI={10.1046/j.1365-2672.2000.01146.x}, abstractNote={The Lactobacillus acidophilus complex includes Lact. acidophilus, Lactobacillus amylovorus, Lactobacillus crispatus, Lactobacillus gallinarum, Lactobacillus gasseri and Lactobacillus johnsonii. The objective of this work was to develop a rapid and definitive DNA sequence‐based identification system for unknown isolates of the Lact. acidophilus complex. A ≈500 bp region of the 16S rRNA gene, which contained the V1 and V2 variable regions, was amplified from the isolates by the polymerase chain reaction. The sequence of this region of the 16S rRNA gene from the type strains of the Lact. acidophilus complex was sufficiently variable to allow for clear differentiation amongst each of the strains. As an initial step in the characterization of potentially probiotic strains, this technique was successfully used to identify a variety of unknown human intestinal isolates. The approach described here represents a rapid and definitive method for the identification of Lact. acidophilus complex members.}, number={3}, journal={JOURNAL OF APPLIED MICROBIOLOGY}, author={Kullen, MJ and Sanozky-Dawes, RB and Crowell, DC and Klaenhammer, TR}, year={2000}, month={Sep}, pages={511–516} } @inbook{kullen_klaenhammer_1999, title={Genetic modification of intestinal lactobacilli and bifidobacteria}, ISBN={1898486158}, booktitle={Probiotics : a critical review}, publisher={Wymondham, Eng. : Horizon Scientific Press}, author={Kullen, M. J. and Klaenhammer, T. R.}, year={1999}, pages={65} }