Works (53)
Updated: September 13th, 2024 12:57
2024 journal article
A primordial DNA store and compute engine
Nature Nanotechnology, 8.
Sources: Crossref, NC State University Libraries, Web Of Science, ORCID
Added: August 25, 2024
2024 chapter
Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities
In Methods in Molecular Biology (pp. 23–55).
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2024 journal article
Single-Cell Assessment of Human Stem Cell-Derived Mesolimbic Models and Their Responses to Substances of Abuse
Organoids, 3(2), 126–147.
open access via www.mdpi.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: August 22, 2024
2024 chapter
Summary of ChIP-Seq Methods and Description of an Optimized ChIP-Seq Protocol
In Methods in Molecular Biology (pp. 419–447).
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2023 review
A molecular assessment of the practical potential of DNA-based computation
[Review of ]. CURRENT OPINION IN BIOTECHNOLOGY, 81.
TL;DR:
A reassessment of the potential of DNA computing systems, which has grown to encompass a diverse array of configurations and form the foundations of neural networks and diagnostic tools that aim to bring molecular computation to practical scales and applications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: September 11, 2023
2023 journal article
Chaetocin disrupts the SUV39H1-HP1 interaction independent of SUV39H1 methyltransferase activity
BIOCHEMICAL JOURNAL, 480(6), 421–432.
MeSH headings : Humans; Methyltransferases / metabolism; Repressor Proteins / metabolism; Histones / genetics; Histones / metabolism; Chromosomal Proteins, Non-Histone / metabolism
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 11, 2023
2023 journal article
FrameD: framework for DNA-based data storage design, verification, and validation
BIOINFORMATICS, 39(10).
Ed(s): J. Kelso
TL;DR:
FrameD fills a void in the tools publicly available to the DNA storage community by providing a modular and extensible framework with support for massive parallelism that will help accelerate the design process of future DNA-based storage systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
9. Industry, Innovation and Infrastructure
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 11, 2023
2023 journal article
Matrigel Tunes H9 Stem Cell-Derived Human Cerebral Organoid Development
Organoids, 2(4), 165–176.
Sources: ORCID, Crossref, NC State University Libraries
Added: August 22, 2024
2023 journal article
Profiling transcriptomic responses of human stem cell-derived medium spiny neuron-like cells to exogenous phasic and tonic neurotransmitters
MOLECULAR AND CELLULAR NEUROSCIENCE, 126.
author keywords: Human pluripotent stem cells; Medium spiny neuron; Tonic neuromodulation; Neurotransmitter; Gene desensitization; Transcriptome
TL;DR:
The results here suggest future optimizations of human stem cell derived neurons to increase their in vivo relevance and the biological insights that can be garnered from these models.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 28, 2023
2023 journal article
Reactive Oxygen Species Mediate Transcriptional Responses to Dopamine and Cocaine in Human Cerebral Organoids
International Journal of Molecular Sciences, 24(22), 16474.
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: ORCID, Crossref, NC State University Libraries
Added: August 22, 2024
2023 journal article
Reimaging DNA as the Code of (More Than) Life
Chemical Engineering Progress, 119(11), 34–37.
Source: NC State University Libraries
Added: September 13, 2024
2022 journal article
DINOS: Data INspired Oligo Synthesis for DNA Data Storage
ACM JOURNAL ON EMERGING TECHNOLOGIES IN COMPUTING SYSTEMS, 18(3).
author keywords: DNA information storage; DNA synthesis; DNA assembly algorithms
TL;DR:
The approach offers greater density by up to 80% over a prior general purpose gene assembly technique, and in an analysis of synthesis costs, it is estimated that DINOS is as 105× cheaper than de novo synthesis.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 14, 2022
2022 journal article
Human Pluripotent Stem Cell-Derived Medium Spiny Neuron-like Cells Exhibit Gene Desensitization
CELLS, 11(9).
author keywords: dopamine; gene desensitization; acute and chronic; RNA-seq; human embryonic stem cell; medium spiny neuron
MeSH headings : Dopamine; Humans; Neurites; Neurons / physiology; Pluripotent Stem Cells; Receptors, Dopamine
TL;DR:
The dynamic responses of human stem cell-derived medium spiny neuron-like cells (hMSN- like cells) to dopamine are mapped and it is shown that these human neurons can reflect and capture cellular desensitization to chronic versus acute administration of dopamine.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science; OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: May 23, 2022
2022 journal article
Modified Histone Peptides Linked to Magnetic Beads Reduce Binding Specificity
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23(3).
author keywords: yeast surface display; histone post-translational modifications; affinity reagents; protein engineering; chromodomain; binder; reader; antibody; epigenome engineering; synthetic biology
MeSH headings : HEK293 Cells; Histone Code; Histones / metabolism; Humans; Jurkat Cells; K562 Cells; Protein Array Analysis; Protein Binding; Protein Engineering; Protein Processing, Post-Translational; Saccharomyces cerevisiae
TL;DR:
Specific experimental conditions that affect binding specificities of post-translationally modified histones in classic protein engineering platforms are described and likely explain the relative difficulty with histone targets in these platforms.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: February 21, 2022
2022 chapter book
Yeast Display Guided Selection of pH-Dependent Binders
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2021 review
DNA stability: a central design consideration for DNA data storage systems
[Review of ]. NATURE COMMUNICATIONS, 12(1).
MeSH headings : DNA / genetics; Data Systems; Information Storage and Retrieval
TL;DR:
It is proposed that the stability of DNA should be a key consideration in how it is used for data storage and pose specific design configurations and scenarios for future systems that best leverage the considerable advantages of DNA storage.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
7. Affordable and Clean Energy
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: April 19, 2021
2021 journal article
Effects of early geometric confinement on the transcriptomic profile of human cerebral organoids
BMC BIOTECHNOLOGY, 21(1).
author keywords: Human cerebral organoids; Neurodevelopment; Agarose microwells; Microfabrication; RNAseq; Mechanobiology
MeSH headings : Brain; Cell Differentiation; Gene Expression Profiling; Humans; Organoids; Transcriptome
TL;DR:
A role for spatial factors in brain region specification during hCO development is suggested and this work observed that non-spherical shapes showed signs of altered neurodevelopmental kinetics and favored the development of medial ganglionic eminence-associated brain regions and cell types over cortical regions.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: October 26, 2021
2021 journal article
Evaluation of UBE3A antibodies in mice and human cerebral organoids
SCIENTIFIC REPORTS, 11(1).
MeSH headings : Angelman Syndrome / genetics; Angelman Syndrome / pathology; Animals; Antibodies / genetics; Antibodies / immunology; Autistic Disorder / genetics; Autistic Disorder / pathology; Cerebellum / cytology; Disease Models, Animal; Genomic Imprinting; Humans; Mice; Neural Stem Cells / metabolism; Neurons / metabolism; Neurons / pathology; Organoids / cytology; Pluripotent Stem Cells; Ubiquitin-Protein Ligases / genetics; Ubiquitin-Protein Ligases / immunology
TL;DR:
While four antibodies show specific localization patterns in both mouse brain sections and human cerebral organoids, these antibodies varied significantly in background signals and staining patterns in undifferentiated human pluripotent stem cells.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 19, 2021
2021 journal article
Mapping the dynamic transfer functions of eukaryotic gene regulation
CELL SYSTEMS, 12(11), 1079-+.
MeSH headings : Chromatin / genetics; Eukaryota / genetics; Gene Expression Regulation / genetics; Promoter Regions, Genetic / genetics; Signal Transduction
TL;DR:
It is determined that chromatin state could be used to tune mutual information and expression levels, as well as completely alter the input-output transfer function of the promoter, which unlocks the information-rich content of eukaryotic gene regulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: January 31, 2022
2021 journal article
Mapping the residue specificities of epigenome enzymes by yeast surface display
Cell Chemical Biology, 28(12), 1772–1779.e4.
MeSH headings : E1A-Associated p300 Protein / genetics; E1A-Associated p300 Protein / metabolism; Epigenome / genetics; Humans; Saccharomyces cerevisiae / metabolism
TL;DR:
The results show that using yeast surface display to study histone modifications is a useful tool that can advance the understanding of chromatin biology by enabling efficient interrogation of the complexity of epigenome modifications.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, Crossref
Added: January 3, 2022
2021 journal article
Promiscuous molecules for smarter file operations in DNA-based data storage
NATURE COMMUNICATIONS, 12(1).
MeSH headings : Computer Simulation; DNA / chemistry; DNA Primers / chemistry; Databases, Nucleic Acid; High-Throughput Nucleotide Sequencing; Image Processing, Computer-Assisted / methods; Information Storage and Retrieval / methods; Real-Time Polymerase Chain Reaction / methods; Software; Temperature; Thermodynamics
TL;DR:
The authors leverage thermodynamics to control the access of different subsets of data in a file and provide an argument for the substantial and practical economic benefit of this generalizable strategy to organize data.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: July 12, 2021
2021 article
Voices of biotech research
Annabi, N., Baker, M., Boettiger, A., Chakraborty, D., Chen, Y., Corbett, K. S., … Wang, J. (2021, March). NATURE BIOTECHNOLOGY, Vol. 39, pp. 281–286.
MeSH headings : Biomedical Research; Biotechnology; Humans; Research Personnel
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: April 5, 2021
2020 review
Capturing complex epigenetic phenomena through human multicellular systems
[Review of ]. CURRENT OPINION IN BIOMEDICAL ENGINEERING, 16, 34–41.
author keywords: Epigenetics; Organoids; Development; Aging; 3D; Multicellular
TL;DR:
Recent work is highlighted that demonstrates how multicellular systems provide a natural approach to capture complex epigenetic phenomena through more closely matching the in vivo environment and through the intrinsic nature of multi-cellular systems being able to generate and model multiple distinct cellular states, all within one system.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: June 10, 2021
2020 journal article
Dynamic and scalable DNA-based information storage
NATURE COMMUNICATIONS, 11(1).
MeSH headings : Algorithms; Bacteriophage T7 / genetics; DNA / genetics; DNA, Single-Stranded / genetics; Gene Expression Regulation, Viral; Genetic Code; Models, Genetic; Promoter Regions, Genetic / genetics; Transcription, Genetic
TL;DR:
It is shown that a simple architecture comprised of a T7 promoter and a single-stranded overhang domain (ss-dsDNA), can unlock dynamic DNA-based information storage with powerful capabilities and advantages.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: July 13, 2020
2020 journal article
Human Cerebral Organoids Reveal Early Spatiotemporal Dynamics and Pharmacological Responses of UBE3A
STEM CELL REPORTS, 15(4), 845–854.
MeSH headings : Angelman Syndrome / pathology; Cell Line; Cell Nucleus / drug effects; Cell Nucleus / metabolism; Cerebrum / metabolism; Genomic Imprinting / drug effects; Humans; Neurons / drug effects; Neurons / metabolism; Organoids / drug effects; Organoids / metabolism; Time Factors; Topoisomerase Inhibitors / pharmacology; Ubiquitin-Protein Ligases / metabolism
TL;DR:
Human cerebral organoids are established as an important model for studying UBE3A and motivates their broader use in understanding complex neurodevelopmental disorders.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
4. Quality Education
(OpenAlex)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: November 9, 2020
2019 journal article
Driving the Scalability of DNA-Based Information Storage Systems
ACS SYNTHETIC BIOLOGY, 8(6), 1241–1248.
author keywords: synthetic biology; DNA storage; information storage; nested architecture; file access; DNA sequencing
MeSH headings : DNA / chemistry; DNA / genetics; Databases, Nucleic Acid; High-Throughput Nucleotide Sequencing; Information Storage and Retrieval / methods; Sequence Analysis, DNA / methods; Synthetic Biology / methods
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries, ORCID
Added: July 15, 2019
2019 article
Human cerebral organoids capture the spatiotemporal complexity and disease dynamics of UBE3A
Sen, D., Voulgaropoulos, A., Drobna, Z., & Keung, A. J. (2019, August 22).
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Source: ORCID
Added: September 1, 2024
2019 journal article
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
NATURE COMMUNICATIONS, 10.
MeSH headings : Base Sequence; CRISPR-Associated Proteins / metabolism; Clustered Regularly Interspaced Short Palindromic Repeats / genetics; DNA / genetics; Endonucleases / metabolism; Gene Library; Genetic Techniques; HEK293 Cells; Humans; Nucleic Acid Conformation; Plasmids / metabolism; RNA / biosynthesis; RNA, Messenger / genetics; RNA, Messenger / metabolism; Saccharomyces cerevisiae / metabolism
TL;DR:
A modular, one-pot scheme called CRATES to construct CRISPR arrays and array libraries that can facilitate diverse multiplexing applications and help identify factors impacting crRNA biogenesis is presented.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: July 22, 2019
2018 chapter book
Chromatin Immunoprecipitation in Human and Yeast Cells
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2018 chapter book
Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2018 journal article
Engineering Epigenetic Regulation Using Synthetic Read-Write Modules
CELL, 176(1-2), 227-+.
TL;DR:
This work established an orthogonal epigenetic regulatory system in mammalian cells using N6-methyladenine (m6A), a DNA modification not commonly found in metazoan epigenomes, and used this system and mathematical models to construct regulatory circuits that induce m6A-dependent transcriptional states, promote their spatial propagation, and maintain epigenetic memory of the states.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
4. Quality Education
(OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: January 28, 2019
2018 journal article
The untapped information content of the epigenome
Chemical Engineering Progress, 114(9), 50–54.
Source: NC State University Libraries
Added: September 13, 2024
2016 article
A unifying model of epigenetic regulation
Keung, A. J., & Khalil, A. S. (2016, February 12). SCIENCE, Vol. 351, pp. 661–662.
MeSH headings : Animals; Chromatin / metabolism; DNA Methylation; Gene Silencing; Histones / metabolism; Humans
TL;DR:
By studying how individual chromatin regulators (CRs) operate to produce distinct gene expression outputs within individual cells, Bintu et al. find that chromatin's complexity can be reduced into an elegant and unifying model of gene regulation.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2016 review
The epigenome: the next substrate for engineering
[Review of ]. GENOME BIOLOGY, 17.
MeSH headings : Chromatin / genetics; Epigenesis, Genetic; Epigenomics; Genome, Human; Humans
TL;DR:
The design and state of epigenome editing tools are reviewed, highlighting the unique regulatory properties afforded by these systems.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018
2015 journal article
Chromatin regulation at the frontier of synthetic biology
Nature Reviews Genetics, 16(3), 159–171.
MeSH headings : Animals; Cell Engineering / methods; Chromatin / chemistry; Chromatin / metabolism; Epigenesis, Genetic; Genome; Histones / chemistry; Histones / metabolism; Humans; Plants / genetics; Plants / metabolism; RNA, Untranslated / chemistry; RNA, Untranslated / metabolism; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Synthetic Biology; Systems Biology
TL;DR:
These studies lay the foundation for biomedical and biotechnological engineering applications that could take advantage of the unique combinatorial and spatiotemporal layers of chromatin regulation to create synthetic systems of unprecedented sophistication.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: February 24, 2020
2014 journal article
Using Targeted Chromatin Regulators to Engineer Combinatorial and Spatial Transcriptional Regulation
Cell, 158(1), 110–120.
MeSH headings : Chromatin / metabolism; Gene Expression Regulation, Fungal; Gene Regulatory Networks; Genes, Reporter; Saccharomyces cerevisiae / genetics; Saccharomyces cerevisiae / metabolism; Saccharomyces cerevisiae Proteins / chemistry; Saccharomyces cerevisiae Proteins / metabolism; Transcription, Genetic
TL;DR:
A synthetic biology approach to decipher the complexity of chromatin regulation by studying emergent transcriptional behaviors from engineered combinatorial, spatial, and temporal patterns of individual CRs.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2013 journal article
Pan-neuronal maturation but not neuronal subtype differentiation of adult neural stem cells is mechanosensitive
Scientific Reports, 3(1).
MeSH headings : Adult Stem Cells / cytology; Adult Stem Cells / metabolism; Animals; Brain / metabolism; Cell Differentiation / physiology; Cell Lineage; Cellular Microenvironment / physiology; Female; Gene Expression; Gene Expression Regulation, Developmental; Neural Stem Cells / cytology; Neural Stem Cells / metabolism; Neurons / classification; Neurons / cytology; Neurons / metabolism; Rats; Time Factors; Tubulin / genetics
TL;DR:
C cultured adult neural stem cells on variable stiffness ECMs under conditions that promote neuronal fate commitment for extended time periods suggest that a wide range of stiffnesses fully permit pan-neuronal NSC differentiation, that an intermediate stiffness optimizes expression of pan-Neuronal genes and that stiffness does not impact commitment to particular neuronal subtypes.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2012 journal article
A Genetic Strategy for the Dynamic and Graded Control of Cell Mechanics, Motility, and Matrix Remodeling
Biophysical Journal, 102(3), 434–442.
MeSH headings : Biomechanical Phenomena; Cell Line; Cell Movement / drug effects; Cell Movement / genetics; Collagen / metabolism; Extracellular Matrix / drug effects; Extracellular Matrix / genetics; Extracellular Matrix / metabolism; Gene Expression Regulation / drug effects; Gene Expression Regulation / genetics; Genetic Engineering / methods; Humans; Mechanical Phenomena; Mechanotransduction, Cellular / drug effects; Mechanotransduction, Cellular / genetics; Mutation; Promoter Regions, Genetic / genetics; Tetracycline / pharmacology
TL;DR:
It is demonstrated that soft ECMs render cells maximally sensitive to changes in RhoA activity, and that by modulating the ability of cells to engage and contract soft ECM, the strategy enables the physical interactions between cells and the ECM and thereby dictate how cells respond to matrix properties.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2012 journal article
Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells
Integrative Biology, 4(9), 1049–1058.
MeSH headings : Cell Culture Techniques / methods; Cell Differentiation / physiology; Dopaminergic Neurons / cytology; Extracellular Matrix / physiology; Flow Cytometry; Humans; Microscopy, Atomic Force; Microscopy, Phase-Contrast; Neurogenesis / physiology; Pluripotent Stem Cells / cytology; Stress, Mechanical
TL;DR:
It is found that increasing extracellular matrix stiffness in vitro increases hPSC cell and colony spread area but does not alter self-renewal, in contrast to past studies with mESCs, and softer ECMs with stiffnesses similar to that of neural tissue promote the generation of early neural ectoderm.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2011 journal article
An Evolved Adeno-associated Viral Variant Enhances Gene Delivery and Gene Targeting in Neural Stem Cells
Molecular Therapy, 19(4), 667–675.
MeSH headings : Animals; Cells, Cultured; Chromatography; Dependovirus / genetics; Female; Fluorescent Antibody Technique; Gene Transfer Techniques; Neural Stem Cells / virology; Polymerase Chain Reaction; Rats; Rats, Inbred F344
TL;DR:
This work applied directed evolution to create a "designer" AAV vector with enhanced delivery efficiency for neural stem cells (NSCs), and a novel AAV variant, carrying an insertion of a selected peptide sequence on the surface of the threefold spike within the heparin-binding site, emerged from this evolution.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2011 journal article
Progress and Prospects for Stem Cell Engineering
Annual Review of Chemical and Biomolecular Engineering, 2(1), 479–502.
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2011 journal article
Rho GTPases Mediate the Mechanosensitive Lineage Commitment of Neural Stem Cells
STEM CELLS, 29(11), 1886–1897.
author keywords: Neural stem cells; Cellular mechanotransduction; Rho GTP-binding proteins; Adipose stem cells; Extracellular matrix; Elastic modulus
MeSH headings : Animals; Apoptosis / genetics; Apoptosis / physiology; Cell Differentiation / genetics; Cell Differentiation / physiology; Cell Proliferation; Cells, Cultured; Extracellular Matrix / metabolism; Female; Fluorescent Antibody Technique; Mechanotransduction, Cellular / genetics; Mechanotransduction, Cellular / physiology; Microscopy, Atomic Force; Neural Stem Cells / cytology; Neural Stem Cells / metabolism; Neurogenesis / genetics; Neurogenesis / physiology; Rats; Rats, Inbred F344; cdc42 GTP-Binding Protein / genetics; cdc42 GTP-Binding Protein / metabolism; rho GTP-Binding Proteins / genetics; rho GTP-Binding Proteins / metabolism; rhoA GTP-Binding Protein / genetics; rhoA GTP-Binding Protein / metabolism
TL;DR:
These results establish Rho GTPase‐based mechanotransduction and cellular stiffness as biophysical regulators of NSC fate in vitro and RhoA as an important regulatory protein in the hippocampal stem cell niche.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2010 journal article
A Genetic Strategy for Graded and Dynamic Control of Cell-Matrix Mechanobiology
Biophysical Journal, 98(3), 730a.
TL;DR:
Genetically engineered stable cell lines are engineered in which they can precisely and dynamically alter the mechanobiological behavior of living cells by varying the activity of signal transduction proteins, such as RhoA GTPase, using constitutively active and dominant negative mutants under the control of a tetracycline-repressible promoter.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2010 journal article
Akt Increases Sox2 Expression in Adult Hippocampal Neural Progenitor Cells, but Increased Sox2 Does Not Promote Proliferation
Stem Cells and Development, 20(7), 1153–1161.
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2010 journal article
Extracellular matrix rigidity modulates neuroblastoma cell differentiation and N-myc expression
Molecular Cancer, 9(1).
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(OpenAlex)
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2010 journal article
Mechanotransductive Engineering of Neural Stem Cell Behavior
Biophysical Journal, 98(3), 404a–405a.
TL;DR:
It is demonstrated that ECM-derived mechanical signals can act with Rho GTPases to regulate NSC stiffness and differentiation, establishing RhoGTPase-based mechanotransduction and cellular stiffness as novel regulators of NSC behavior.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2010 journal article
Presentation Counts: Microenvironmental Regulation of Stem Cells by Biophysical and Material Cues
Annual Review of Cell and Developmental Biology, 26(1), 533–556.
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2009 journal article
Biophysics and dynamics of natural and engineered stem cell microenvironments
Wiley Interdisciplinary Reviews: Systems Biology and Medicine, 2(1), 49–64.
MeSH headings : Adult; Animals; Biomechanical Phenomena / physiology; Biophysics / methods; Biophysics / trends; Cell Culture Techniques / methods; Environment; Humans; Kinetics; Models, Biological; Stem Cells / cytology; Stem Cells / metabolism; Stem Cells / physiology; Tissue Engineering / methods
TL;DR:
How microenvironmental characteristics of the microenvironment have been shown to regulate stem cell fate are reviewed and future research directions that may help expand the current understanding of stem cell biology and aid its application to regenerative medicine are discussed.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2008 journal article
Substrate Modulus Directs Neural Stem Cell Behavior
Biophysical Journal, 95(9), 4426–4438.
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2006 journal article
Formation of Surface-Bound Acyl Groups by Reaction of Acyl Halides on Ge(100)−2×1
The Journal of Physical Chemistry B, 110(9), 4115–4124.
Sources: Crossref, NC State University Libraries
Added: August 25, 2024
2006 journal article
Thermal Control of Amide Product Distributions at the Ge(100)-2×1 Surface
The Journal of Physical Chemistry C, 111(1), 411–419.
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2005 journal article
Carboxylic Acid Chemistry at the Ge(100)-2 × 1 Interface: Bidentate Bridging Structure Formation on a Semiconductor Surface
Journal of the American Chemical Society, 128(3), 770–779.
TL;DR:
Investigation of the reactions of acetic acid, acetic-d3 acid-d, and formic acid with the Ge(100)-2 x 1 surface suggests that the O-H dissociation pathway is significantly favored, both kinetically and thermodynamically, over other potential reaction pathways.
(via Semantic Scholar)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
2005 journal article
Tertiary amide chemistry at the Ge(100)-2×1 surface
Surface Science, 599(1-3), 41–54.
author keywords: density functional calculations; vibrational spectroscopies; germanium; organic molecules; organo-functionalization of surfaces; desorption; amide; Ge(100)
Sources: Crossref, NC State University Libraries
Added: August 28, 2020
Employment
Updated: September 25th, 2019 12:36
North Carolina State University Raleigh, NC, US
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