@article{isleib_wilson_novitzky_2006, title={Partial dominance, pleiotropism, and epistasis in the inheritance of the high-oleate trait in peanut}, volume={46}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2005.09-0313}, abstractNote={ABSTRACT}, number={3}, journal={CROP SCIENCE}, author={Isleib, TG and Wilson, RF and Novitzky, WP}, year={2006}, pages={1331–1335} } @article{isleib_rice_mozingo_copeland_graeber_novitzky_pattee_sanders_mozingo_coker_2006, title={Registration of 'Brantley' peanut}, volume={46}, ISSN={["0011-183X"]}, DOI={10.2135/cropsci2005.12.0492}, abstractNote={‘Brantley’ (Reg. no. CV-86, PI 642026) is a large-seeded virginia-type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) cultivar with high oleic fatty acid content in its seed oil, essentially derived from the ‘NC 7’ cultivar (Wynne et al., 1979). Brantley was tested under the experimental designation N00090ol and was released jointly by the North Carolina Agric. Res. Service (NCARS) and the USDA-ARS in 2005. Brantley was tested by the NCARS, by the Virginia Agric. Exp. Stn. (VAES), and five other state agricultural experiment stations participating in the Uniform Peanut Performance Tests (UPPT). Brantley is named in honor of the late Ms. Peggy Y. Brantley, long-time office administrator to the peanut breeding and genetics programs at North Carolina State Univ. (NCSU). Brantley is a virginia market type cultivar possessing alternate branching pattern, intermediate runner growth habit, medium green foliage, large seeds with tan testa averaging 895 mg seed, approximately 65% jumbo pods and 24% fancy pods, and extra large kernel content of approximately 50%. Brantley was developed by backcrossing the high-oleic trait patented by the University of Florida (Norden et al., 1987; Moore and Knauft, 1989; Knauft et al., 1993) into the NC 7 cultivar from Florida line F435 (Moore and Knauft, 1989). In the backcrossing steps, F4:5 progenies or individual F2 seeds were analyzed for fatty acid profiles using gas chromatography (Zeile et al., 1993), and high-oleic families or seeds were selected. BC4F2:3 families were grown in a replicated preliminary yield test in 1999. N00090ol was numbered in 2000 on entry into the NCSUAdvanced Yield Test series. Agronomic performance of Brantley has been evaluated in 16 trials conducted by the NCARS breeding program over 5 yr and 24 trials (including early and late diggings as separate trials) in the joint VAES-NCARS Peanut Variety and Quality Evaluation (PVQE) program over 3 yr (Coker and Mozingo, 2004, 2005). Brantley was also tested in the Uniform Peanut Performance Test series conducted at nine sites in seven states in 2003 (Branch et al., 2004). Because it was essentially derived from NC 7 by backcrossing, most characteristics of Brantley are similar to those of NC 7. The following comparisons are based on results from the PVQE program except as noted. Compared with NC 7, Brantley has similar pod yield (4479 vs. 4492 kg ha,ns), sound mature kernel content (67%), and meat content (73%), but more jumbo pods (64 vs. 58%, P , 0.05), fewer fancy size pods (24 vs. 29%, P, 0.05), more extra large kernels (ELK) (53 vs. 48%, P , 0.05), and greater jumbo pod brightness (44.5 vs. 43.5 Hunter L score, P , 0.01) (Isleib et al., 1997) and average pod brightness (43.9 vs. 43.0 Hunter L score, P , 0.01). The ratio of oleic to linoleic fatty acid of Brantley was greater than that of NC 7 (27.77 vs. 2.61, P , 0.01). In the NCSU trials, Brantley had greater brightness of jumbo pods than NC 7 (46.3 vs. 45.5 Hunter L score, P, 0.01), greater average pod brightness (45.1 vs. 44.4 Hunter L score, P , 0.05), and greater ELK content (46 vs. 43%, P , 0.01). Flavor attributes of roasted sound mature kernel samples from eight NCSU trials were evaluated by a trained sensory panel under the direction of USDA personnel. Averaged across eight samples taken from NCARS trials in 2000 and 2002 and adjusted to common values of roast color and fruity attribute (Pattee and Giesbrecht, 1990), the roasted peanut, sweet, bitter, and astringent attributes of flavor in Brantley were not different from those in NC 7, the flavor standard for the virginia market type. Averaged across ELK samples from nine UPPT locations from 2003 (USDA-ARS, 2004), Brantley was not significantly different from NC 7 for intensity of roasted peanut [4.41 vs. 4.61 flavor intensity units (fiu), ns], sweet (1.98 vs. 2.02 fiu, ns), or bitter (3.07 vs. 2.97 fiu, ns). Although it was not developed specifically to carry any particular disease resistance, Brantley was evaluated for resistance to diseases common to the Virginia-Carolina region. Because it was essentially derived from NC 7 by backcrossing, it was found to have the same susceptibilities to disease as NC 7. Brantley’s reaction to early leafspot (caused by Cercospora arachidicola S. Hori) was evaluated from 2001 through 2004 in four field trials with no application of leafspot fungicide during the entire season. Defoliation was rated on a proportional scale of 1 (no defoliation) to 9 (complete defoliation) in late September or early October each year, and yield was measured. Brantley was not significantly different from NC 7 in defoliation (7.0 vs. 6.8 defoliation score, ns) or yield (2380 vs. 2346 kg ha, ns). Brantley’s reactions to Cylindrocladium black rot (CBR) {caused by Cylindrocladium parasiticum Crous, Wingfield & Alfenas [syn. C. crotalariae (Loos) D.K. Bell & Sobers]} and to Sclerotinia blight (caused by Sclerotinia minor Jagger) were evaluated by the NCSU breeding project in four replicated tests on naturally infested soils with no chemical control of the soilborne diseases. Brantley was not different from NC 7 in incidence of CBR (37 vs. 33%, ns) or Sclerotinia blight (28 vs. 44%, ns). Brantley’s reaction to Tomato spotted wilt virus (TSWV) was evaluated from 2001 through 2004 in four field trials with seeds spaced 50 cm apart and no application of insecticides to control the thrips (Frankliniella fusca Hinds), the vector of the virus. Brantley was not different from NC 7 in incidence of TSWV symptoms (44 vs. 36%, ns). Like its recurrent parent NC 7, Brantley should be considered susceptible to all four of these diseases. Brantley is adapted to the Virginia–Carolina peanut production area but also has performed well in the southeastern US production area including Georgia, Florida, and Alabama. Breeder seed of Brantley will be maintained by the N.C. Agricultural Research Service, Box 7643, N.C. State University, Raleigh, NC 27695–7643. Foundation seed will be distributed by the N.C. Foundation Seed Producers, Inc., 8220 Riley Hill Rd., Zebulon, NC 27597. The N.C. Agricultural Research Service will provide small (50–100 seed) samples to research organizations for research purposes. An application is pending for protection of Brantley under the U.S. Plant Variety Protection Act as amended in 1994, under which Brantley may be sold only as a class of Certified seed. The high-oleic trait is protected by U.S. Patents (No. 5922,390, 6063,984, and 6121,472) issued to theUniv. of Florida by whose permission the trait was used.}, number={5}, journal={CROP SCIENCE}, author={Isleib, T. G. and Rice, P. W. and Mozingo, R. W., II and Copeland, S. C. and Graeber, J. B. and Novitzky, W. P. and Pattee, H. E. and Sanders, T. H. and Mozingo, R. W. and Coker, D. L.}, year={2006}, pages={2309–2311} } @article{xue_isleib_payne_novitzky_obrian_2005, title={Aflatoxin production in peanut lines selected to represent a range of linoleic acid concentrations}, volume={68}, ISSN={["1944-9097"]}, DOI={10.4315/0362-028X-68.1.126}, abstractNote={To determine whether concentrations of linoleate in peanut (Arachis hypogaea L.) seed oil could be used to predict an ability to support aflatoxin production, seeds of genotypes representing a range of linoleate content were inoculated with Aspergillus flavus Link ex Fries and assayed for aflatoxin content. Seeds were blanched and quartered, inoculated with conidia of A. flavus, placed on moistened filter paper in petri dishes, and incubated for 8 days at 28 degrees C. Multiple regression analysis was used to account for the variation among lines with the use of fatty acid concentrations as independent variables. In test 1, linoleate accounted for 39 to 44% of the variation among lines for aflatoxin B1 and B2 and total aflatoxin (26 to 27% after log transformation). Oleate accounted for substantial additional variation (27 to 29%) among lines (20 to 23% after log transformation). Other fatty acids accounted for small fractions of among-line variation. In test 2, linoleate accounted for about 35 to 44% of the variation among entries across traits (29 to 37% for log-transformed data); arachidate accounted for 19 to 29% (27 to 33% after log transformation). Eicosenoate accounted for a small part of the total entry variation. In both experiments, residual variation among entries was significant. Low-linoleate lines consistently contained more aflatoxin, whereas normal- to high-linoleate lines contained variable amounts. Although fatty acid concentrations accounted for significant portions of genetic variation, it is not practical to use them as predictors for susceptibility to aflatoxin contamination, especially for lines in the normal range for oleate and linoleate.}, number={1}, journal={JOURNAL OF FOOD PROTECTION}, author={Xue, HQ and Isleib, TG and Payne, GA and Novitzky, WF and Obrian, G}, year={2005}, month={Jan}, pages={126–132} } @article{tang_novitzky_griffin_huber_dewey_2005, title={Oleate desaturase enzymes of soybean: evidence of regulation through differential stability and phosphorylation}, volume={44}, ISSN={["1365-313X"]}, DOI={10.1111/j.1365-313X.2005.02535.x}, abstractNote={Summary}, number={3}, journal={PLANT JOURNAL}, author={Tang, GQ and Novitzky, WP and Griffin, HC and Huber, SC and Dewey, RE}, year={2005}, month={Nov}, pages={433–446} } @article{xue_isleib_payne_wilson_novitzky_g o'brian_2003, title={Comparison of aflatoxin production in normal- and high-oleic backeross-derived peanut lines}, volume={87}, ISSN={["1943-7692"]}, DOI={10.1094/PDIS.2003.87.11.1360}, abstractNote={ The effect of the high-oleate trait of peanut on aflatoxin production was tested by comparing normal oleic lines with high-oleic backcross-derived lines. Seeds were blanched, quartered, and inoculated with Aspergillus flavus conidia, placed on moistened filter paper in petri dishes, and incubated for 8 days. In one experiment, dishes were stacked in plastic bags in a Latin square design with bags and positions in stacks as blocking variables. High-oleic lines averaged nearly twice as much aflatoxin as normal lines. Background genotype had no significant effect on aflatoxin content, and interaction between background genotype and oleate level was not detected. In a second experiment, dishes were arranged on plastic trays enclosed in plastic bags and stacked with PVC spacers between trays. Fungal growth and aflatoxin production were greater than in the first experiment. Background genotype, oleate level, and their interaction were significant. The mean of high-oleic lines was almost twice that of normal lines, but the magnitude of the difference varied with background genotype. Special care should be taken with high-oleic lines to prevent growth of Aspergillus spp. and concomitant development of aflatoxin contamination. }, number={11}, journal={PLANT DISEASE}, author={Xue, HQ and Isleib, TG and Payne, GA and Wilson, RF and Novitzky, WP and G O'Brian}, year={2003}, month={Nov}, pages={1360–1365} } @article{pantalone_wilson_novitzky_burton_2002, title={Genetic regulation of elevated stearic acid concentration in soybean oil}, volume={79}, ISSN={["1558-9331"]}, DOI={10.1007/s11746-002-0520-8}, abstractNote={Abstract}, number={6}, journal={JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY}, author={Pantalone, VR and Wilson, RF and Novitzky, WP and Burton, JW}, year={2002}, month={Jun}, pages={549–553} } @article{wilson_marquardt_novitzky_burton_wilcox_dewey_2001, title={Effect of alleles governing 16 : 0 concentration on glycerolipid composition in developing soybeans}, volume={78}, ISSN={["1558-9331"]}, DOI={10.1007/s11746-001-0264-5}, abstractNote={Abstract}, number={4}, journal={JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY}, author={Wilson, RF and Marquardt, TC and Novitzky, WP and Burton, JW and Wilcox, JR and Dewey, RE}, year={2001}, month={Apr}, pages={329–334} }