Doris H. D'Souza

Works (7)

Updated: July 5th, 2023 16:00

2007 journal article

Genotypes, serotypes, and antibiotic resistance profiles of Salmonella isolated from commercial North Carolina Turkey farms

JOURNAL OF FOOD PROTECTION, 70(6), 1328–1333.

By: F. Santos  n, D. D'Souza*, L. Jaykus n, P. Ferket n  & B. Sheldon n

MeSH headings : Age Factors; Animals; Anti-Bacterial Agents / pharmacology; Consumer Product Safety; DNA, Bacterial / analysis; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-Field / veterinary; Feces / microbiology; Food Contamination / prevention & control; Food Microbiology; Genotype; Humans; Microbial Sensitivity Tests; North Carolina; Phylogeny; Salmonella / classification; Salmonella / drug effects; Salmonella / genetics; Salmonella / isolation & purification; Salmonella Food Poisoning / prevention & control; Seasons; Serotyping / veterinary; Turkeys / microbiology
TL;DR: Salmonella enterica serotypes, genotypes and AbR patterns were affected by bird age but not by season or farm, and dendogram analysis indicated that sequence variability between serotypes was more significant than within serotypes. (via Semantic Scholar)
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14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Persistence of caliciviruses on enviromnental surfaces and their transfer to food

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 108(1), 84–91.

By: . DH D'Souza, A. Sair*, K. Williams  n, E. Papafragkou n, J. Jean *, C. Moore n, L. Jaykus n

author keywords: norovirus; feline calicivirus; persistence; transfer; food
MeSH headings : Calicivirus, Feline / growth & development; Calicivirus, Feline / isolation & purification; Calicivirus, Feline / physiology; Consumer Product Safety; Environmental Microbiology; Equipment Contamination; Food Contamination / analysis; Food Microbiology; Humans; Norovirus / growth & development; Norovirus / isolation & purification; Norovirus / physiology; RNA, Viral / analysis; RNA, Viral / isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Stainless Steel / analysis; Time Factors; Viral Plaque Assay
TL;DR: This study confirms lengthy NoV persistence on common food preparation surfaces and their ease of transfer, confirming a potential role for environmental contamination in the propagation of viral gastroenteritis. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2004 journal article

Multiplex nucleic acid sequence-based amplification for simultaneous detection of several enteric viruses in model ready-to-eat foods

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(11), 6603–6610.

By: J. Jean n, . DH D'Souza & L. Jaykus n

MeSH headings : Animals; Food Contamination; Hepatitis A virus / genetics; Hepatitis A virus / isolation & purification; Humans; Lettuce / microbiology; Meat Products / microbiology; Norovirus / genetics; Norovirus / isolation & purification; Polymerase Chain Reaction; Self-Sustained Sequence Replication / methods
TL;DR: The multiplex NASBA system provides rapid and simultaneous detection of clinically relevant food-borne viruses in a single reaction tube and may be a promising alternative to reverse transcription-PCR for the detection of viral contamination of foods. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2003 journal article

Nucleic acid sequence based amplification for the rapid and sensitive detection of Salmonella enterica from foods

JOURNAL OF APPLIED MICROBIOLOGY, 95(6), 1343–1350.

By: . DH D'Souza & L. Jaykus n

author keywords: detection; foods; NASBA; Salmonella enterica
MeSH headings : Bacteriological Techniques; Colony Count, Microbial; Feasibility Studies; Food Microbiology; Humans; Nucleic Acid Amplification Techniques / methods; RNA, Bacterial / isolation & purification; Salmonella enteritidis / isolation & purification
TL;DR: The purpose of this study was to apply nucleic acid sequence‐based amplification (NASBA) for the detection of Salmonella enterica serovar Enteritidis in representative foods. (via Semantic Scholar)
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2. Zero Hunger (OpenAlex)
Source: Web Of Science
Added: August 6, 2018

2003 journal article

Transcriptional enhancement of RT-PCR for rapid and sensitive detection of Noroviruses

FEMS MICROBIOLOGY LETTERS, 226(2), 339–345.

By: J. Jean n, . D D'Souza & L. Jaykus n

author keywords: Norovirus; Norwalk-like virus; enteric virus; RT-PCR; transcriptional enhancement
MeSH headings : Environmental Microbiology; Food Microbiology; Norovirus / genetics; Norovirus / isolation & purification; Nucleic Acid Hybridization; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction / methods; Self-Sustained Sequence Replication / methods; Sensitivity and Specificity; Transcription, Genetic
TL;DR: The NASBA-derived primers performed comparably to other broadly reactive GII Norovirus primers with respect to detection limits and shows promise for improved detection sensitivity in instances where very low levels of virus contamination might be anticipated. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2002 journal article

Improved detection of human enteric viruses in foods by RT-PCR

JOURNAL OF VIROLOGICAL METHODS, 100(1-2), 57–69.

By: A. Sair n, . DH D'Souza, C. Moe * & L. Jaykus n

author keywords: Norwalk virus; hepatitis A virus; RNA purity; RT-PCR; foods
MeSH headings : DNA Primers; Food Microbiology; Hepatitis A virus / genetics; Hepatitis A virus / isolation & purification; Humans; Norwalk virus / genetics; Norwalk virus / isolation & purification; RNA, Viral / analysis; Reverse Transcriptase Polymerase Chain Reaction
TL;DR: The methods reported here show promise for rapid, sensitive detection of human enteric viruses in foods as evidenced by the ability to detect viral RNA in food concentrates without prior dilution. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

2002 journal article

Zirconium hydroxide effectively immobilizes and concentrates human enteric viruses

LETTERS IN APPLIED MICROBIOLOGY, 35(5), 414–418.

By: . DH D'Souza & L. Jaykus n

MeSH headings : Hepatitis A virus / growth & development; Hepatitis A virus / isolation & purification; Humans; Hydroxides / pharmacology; Norwalk virus / growth & development; Norwalk virus / isolation & purification; Poliovirus / growth & development; Poliovirus / isolation & purification; Polymerase Chain Reaction; Virus Cultivation / methods; Zirconium / pharmacology
TL;DR: This study uses the use of zirconium hydroxide to concentrate bacteria from clinical, environmental, and food samples to detection of human enteric viruses in foods and environmental samples. (via Semantic Scholar)
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Source: Web Of Science
Added: August 6, 2018

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