Keith Ropp Shockley

Works (14)

Updated: July 5th, 2023 16:00

2007 journal article

Impact of substrate glycoside linkage. and elemental sulfur on bioenergetics, of and hydrogen production by the hyperthermophilic Archaeon Pyrococcus furiosus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(21), 6842–6853.

By: C. Chou n, K. Shockley n, S. Conners n, D. Lewis n, D. Comfort n, M. Adams *, R. Kelly n 

MeSH headings : Archaea; Chemical Phenomena; Chemistry; Gene Expression Regulation, Archaeal; Genome, Archaeal; Glycosides / metabolism; Hot Temperature; Hydrogen / metabolism; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / physiology; Sulfur / metabolism
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2007 journal article

Role of the beta 1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus

JOURNAL OF BACTERIOLOGY, 189(2), 583–590.

By: L. Madding n, J. Michel n, K. Shockley n, S. Conners n, K. Epting n, M. Johnson n, R. Kelly n 

MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Archaeal Proteins / physiology; Calorimetry, Differential Scanning; Genome, Archaeal; Hot Temperature; Molecular Chaperones / genetics; Molecular Chaperones / metabolism; Proteasome Endopeptidase Complex / genetics; Proteasome Endopeptidase Complex / metabolism; Proteasome Endopeptidase Complex / physiology; Protein Subunits / genetics; Protein Subunits / metabolism; Protein Subunits / physiology; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Temperature; Transcription, Genetic
TL;DR: Results indicate that the β1 subunit in the P. furiosus 20S proteasome plays a thermostabilizing role and influences biocatalytic properties, suggesting that β subunit composition is a factor in archaeal proteasom function during thermal stress, when polypeptide turnover is essential to cell survival. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

The Thermotoga maritima phenotype is impacted by syntrophic interaction with Methanococcus jannaschii in hyperthermophilic coculture

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 72(1), 811–818.

By: M. Johnson  n, S. Conners n, C. Montero n, C. Chou n, K. Shockley n & R. Kelly n 

MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Coculture Techniques; DNA, Complementary; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Hot Temperature; Methanococcus / growth & development; Oligonucleotide Array Sequence Analysis; Phenotype; Proteome; Thermotoga maritima / classification; Thermotoga maritima / genetics; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism; Transcription, Genetic
TL;DR: The results demonstrate that syntrophic interactions can impact the transcriptome of heterotrophs in methanogenic coculture, and this factor should be considered in examining the microbial ecology in anaerobic environments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2006 journal article

Transcriptional and biochemical analysis of starch metabolism in the hyperthermophilic archaeon Pyrococcus furiosus

JOURNAL OF BACTERIOLOGY, 188(6), 2115–2125.

By: H. Lee *, K. Shockley n, G. Schut *, S. Conners n, C. Montero n, M. Johnson n, C. Chou n, S. Bridger n ...

MeSH headings : Archaeal Proteins / genetics; Bacterial Proteins / genetics; DNA, Archaeal / chemistry; DNA, Archaeal / genetics; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Glucose / metabolism; Glucosephosphates / metabolism; Glucosyltransferases / genetics; Glycogen Debranching Enzyme System / genetics; Glycoside Hydrolases / genetics; Monosaccharide Transport Proteins / genetics; Oligonucleotide Array Sequence Analysis; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Sequence Analysis, DNA; Starch / metabolism; Substrate Specificity
TL;DR: Starch metabolism in P. furiosus was examined by biochemical analyses in conjunction with global transcriptional response data for cells grown on a variety of glucans, and several hypothetical proteins encoded by three gene clusters are also involved in the transport and processing of alpha-glucan substrates by this organism. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

An expression-driven approach to the prediction of carbohydrate transport and utilization regulons in the hyperthermophilic bacterium Thermotoga maritima

JOURNAL OF BACTERIOLOGY, 187(21), 7267–7282.

By: S. Conners n, C. Montero n, D. Comfort n, K. Shockley n, M. Johnson n, . Chhabra n, R. Kelly n 

MeSH headings : 5' Flanking Region / genetics; ATP-Binding Cassette Transporters / chemistry; ATP-Binding Cassette Transporters / genetics; Biological Evolution; Biological Transport / genetics; Carbohydrate Metabolism; Computational Biology; Gene Duplication; Gene Expression Profiling; Genes, Bacterial; Genes, Regulator; Genomics; Monosaccharide Transport Proteins / genetics; Nucleic Acid Conformation; Oligosaccharides / metabolism; Pentose Phosphate Pathway / genetics; Regulon / physiology; Rhamnose / metabolism; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Xylose / metabolism
TL;DR: The results indicate that T. maritima relies extensively on ABC transporters for carbohydrate uptake, many of which are likely controlled by local regulators responsive to either the transport substrate or a key metabolic degradation product. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Genorne-wide transcriptional variation within and between steady states for continuous growth of the hyperthermophile Thermotoga maritima

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(9), 5572–5576.

By: K. Shockley n, K. Scott*, M. Pysz n, S. Conners n, M. Johnson n, C. Montero n, R. Wolfinger *, R. Kelly n 

TL;DR: Maltose-limited, continuous growth of the hyperthermophile Thermotoga maritima at different temperatures and dilution rates showed that transcriptome-wide variation in gene expression within mechanical steady states was minimal compared to that between steady states, supporting the efficacy of chemostat-based approaches for functional genomics studies. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2005 journal article

Population density-dependent regulation of exopolysaccharide formation in the hyperthermophilic bacterium Thermotoga maritima

MOLECULAR MICROBIOLOGY, 55(3), 664–674.

By: M. Johnson n, C. Montero n, S. Conners n, K. Shockley n, S. Bridger n & R. Kelly n 

MeSH headings : Bacterial Proteins / genetics; Bacterial Proteins / metabolism; Culture Media; Ecosystem; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genome, Archaeal; Genome, Bacterial; Methanococcus / genetics; Methanococcus / growth & development; Methanococcus / metabolism; Oligonucleotide Array Sequence Analysis; Polysaccharides, Bacterial / metabolism; Signal Transduction; Temperature; Thermotoga maritima / growth & development; Thermotoga maritima / metabolism; Transcription, Genetic
TL;DR: Results point to the existence of peptide‐based quorum sensing in hyperthermophilic bacteria and indicate that cellular communication should be considered as a component of the microbial ecology within hydrothermal habitats. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 article

Functional genomics-based studies of the microbial ecology of hyperthermophilic micro-organisms

Johnson, M. R., Montero, C. I., Conners, S. B., Shockley, K. R., Pysz, M. A., & Kelly, R. M. (2004, April). BIOCHEMICAL SOCIETY TRANSACTIONS, Vol. 32, pp. 188–192.

By: M. Johnson n, C. Montero n, S. Conners n, K. Shockley n, M. Pysz n & R. Kelly n 

author keywords: ecology; functional qenomics; hyperthermophile; microarray; mixed culture
MeSH headings : Biofilms; DNA, Complementary / metabolism; Ecology; Gene Transfer Techniques; Genes, Archaeal; Genome; Genome, Archaeal; Microscopy, Fluorescence; Oligonucleotide Array Sequence Analysis; Phylogeny; RNA, Ribosomal, 16S / genetics; Temperature
TL;DR: Functional genomics-based approaches are described, using cDNA microarrays, to gain insight into how ecological features such as biofilm formation, species interaction, and possibly even gene transfer may occur in native environments, as well as to determine what genes or sets of genes may be tied to environmental functionality. (via Semantic Scholar)
UN Sustainable Development Goal Categories
15. Life on Land (Web of Science; OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 review

Transcriptional analysis of biofilm formation processes in the anaerobic, hyperthermophilic bacterium Thermotoga maritima

[Review of ]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 70(10), 6098–6112.

By: M. Pysz n, S. Conners n, C. Montero n, K. Shockley n, M. Johnson n, D. Ward n, R. Kelly n 

MeSH headings : ATP-Binding Cassette Transporters / genetics; ATP-Binding Cassette Transporters / metabolism; Base Sequence; Biofilms / growth & development; Bioreactors; DNA, Bacterial / genetics; Gene Expression Profiling; Genes, Bacterial; Iron / metabolism; Models, Biological; Oligonucleotide Array Sequence Analysis; Oxidative Stress; Polysaccharides, Bacterial / genetics; Polysaccharides, Bacterial / metabolism; Sulfur / metabolism; Temperature; Thermotoga maritima / genetics; Thermotoga maritima / physiology; Thermotoga maritima / ultrastructure; Transcription, Genetic
TL;DR: In addition to revealing insights into the phenotype of sessile T. maritima communities, the methodology developed here can be extended to study other anaerobic biofilm formation processes as well as to examine aspects of microbial ecology in hydrothermal environments. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2004 journal article

Transcriptional analysis of dynamic heat-shock response by the hyperthermophilic bacterium Thermotoga maritima

EXTREMOPHILES, 8(3), 209–217.

By: M. Pysz n, D. Ward n, K. Shockley n, C. Montero n, S. Conners n, M. Johnson n, R. Kelly n 

author keywords: cDNA microarray; groESL; heat shock; hrcA; hyperthermophile; Thermotoga
MeSH headings : Base Sequence; DNA, Bacterial / genetics; Endopeptidases / genetics; Gene Expression; Genome, Bacterial; Heat-Shock Response / genetics; Hot Temperature; Oligonucleotide Array Sequence Analysis; SOS Response, Genetics; Sigma Factor / genetics; Thermotoga maritima / genetics; Thermotoga maritima / metabolism; Transcription, Genetic
TL;DR: The results reported here indicate that, while T. maritima shares core elements of the bacterial heat-shock response with mesophiles, the thermal stress regulatory strategies of this organism differ significantly, and it remains to be elucidated whether these differences are related to thermophilicity or phylogenetic placement. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
15. Life on Land (Web of Science)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Carbohydrate-induced differential gene expression patterns in the hyperthermophilic bacterium Thermotoga maritima

JOURNAL OF BIOLOGICAL CHEMISTRY, 278(9), 7540–7552.

By: . Chhabra, K. Shockley*, S. Conners*, K. Scott*, R. Wolfinger* & R. Kelly* 

MeSH headings : Carbohydrate Metabolism; Carbohydrate Sequence; Carbohydrates / chemistry; Carbon / metabolism; DNA, Complementary / metabolism; Down-Regulation; Gene Expression Regulation, Bacterial; Hydrolases / chemistry; Hydrolases / metabolism; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Thermotoga maritima / metabolism; Up-Regulation
TL;DR: The transcriptional response of T. maritima to specific carbohydrate growth substrates indicated that sugar backbone- and linkage-specific regulatory networks are operational in this organism during the uptake and utilization of carbohydrate substrates, and suggests that this organism is capable of adapting to a variety of growth environments containing carbohydrate growth substrate. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Growth of hyperthermophilic Archaeon Pyrococcus futiosus on chitin involves two family 18 chitinases

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(6), 3119–3128.

By: J. Gao  n, M. Bauer n, K. Shockley n, M. Pysz n & R. Kelly n 

MeSH headings : Amino Acid Sequence; Calorimetry; Chitin / metabolism; Chitinases / chemistry; Chitinases / classification; Chitinases / genetics; Chitinases / metabolism; Culture Media; Hexosaminidases / chemistry; Hexosaminidases / genetics; Hexosaminidases / metabolism; Hot Temperature; Molecular Sequence Data; Pyrococcus furiosus / enzymology; Pyrococcus furiosus / growth & development; Pyrococcus furiosus / metabolism; Recombinant Proteins / metabolism; Sequence Analysis, DNA
TL;DR: Two open reading frames (chiA and chiB) were identified in the genome of P. furiosus, which encodes chitinases with sequence similarity to proteins from the glycosyl hydrolase family 18 in less-thermophilic organisms, indicating that these two enzymes work together to recruit chit in-based substrates for P.furiosus growth. (via Semantic Scholar)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2003 journal article

Heat shock response by the hyperthermophilic archaeon Pyrococcus furiosus

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 69(4), 2365–2371.

By: K. Shockley n, D. Ward n, . Chhabra n, S. Conners n, C. Montero n & R. Kelly n 

MeSH headings : Archaeal Proteins / genetics; Archaeal Proteins / metabolism; Blotting, Northern; Gene Expression Profiling; Gene Expression Regulation, Archaeal; Heat-Shock Response; Oligonucleotide Array Sequence Analysis; Protein Folding; Pyrococcus furiosus / genetics; Pyrococcus furiosus / metabolism; Transcription, Genetic
TL;DR: Collective transcriptional analysis of heat shock response in the hyperthermophilic archaeon Pyrococcus furiosus was examined by using a targeted cDNA microarray in conjunction with Northern analyses and differential gene expression suggests that P.furiosus relies on a cooperative strategy of rescue, proteolysis, and stabilization to cope with polypeptide processing during thermal stress. (via Semantic Scholar)
UN Sustainable Development Goal Categories
14. Life Below Water (OpenAlex)
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

2002 journal article

Regulation of endo-acting glycosyl hydrolases in the hyperthermophilic bacterium Thermotoga maritima grown on glucan- and mannan-based polysaccharides

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(2), 545–554.

By: . Chhabra n, K. Shockley n, D. Ward n & R. Kelly n 

MeSH headings : Amino Acid Sequence; Culture Media; Gene Expression Regulation, Bacterial; Glucans / chemistry; Glucans / metabolism; Glycoside Hydrolases / chemistry; Glycoside Hydrolases / genetics; Glycoside Hydrolases / metabolism; Hot Temperature; Mannans / chemistry; Mannans / metabolism; Molecular Sequence Data; Polysaccharides / chemistry; Polysaccharides / metabolism; Recombinant Proteins / genetics; Recombinant Proteins / metabolism; Sequence Analysis, DNA; Thermotoga maritima / enzymology; Thermotoga maritima / genetics; Thermotoga maritima / growth & development
TL;DR: The genome sequence of the hyperthermophilic bacterium Thermotoga maritima encodes a number of glycosyl hydrolases, and the intracellular localization of Cel5A suggest that the physiological role of this enzyme includes hydrolysis of glucomannan oligosaccharides that are transported following initial hydrolyzing by extracellular glycosidases, such as Man5. (via Semantic Scholar)
UN Sustainable Development Goal Categories
Sources: Web Of Science, NC State University Libraries
Added: August 6, 2018

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