@article{mozdziak_giamario_dibner_mccoy_2004, title={A chicken mRNA similar to heterogeneous nuclear ribonucleoprotein H1}, volume={137}, ISSN={["1879-1107"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0346157313&partnerID=MN8TOARS}, DOI={10.1016/j.cbpc.2003.10.007}, abstractNote={Heterogeneous nuclear ribonucleoproteins are predominantly nuclear RNA-binding proteins that function in a variety of cellular activities. The objective of these experiments was to clone a cDNA for a chicken protein similar to other previously reported heterogeneous ribonucleoproteins for other species. The 5' and 3' ends of the chicken mRNA were cloned using Rapid Amplification of cDNA Ends (RACE). Subsequently, the expression of the mRNA sequence was confirmed via Northern analysis. The deduced amino acid sequence was approximately 86% identical to corresponding regions of human, mouse, or zebrafish proteins similar to heterogeneous nuclear ribonucleoprotein H1. The expression data confirmed the size of the predicted mRNA sequence. The newly identified sequence may be employed in future studies aimed at understanding the role of heterogeneous nuclear ribonucleoproteins in avian species.}, number={1}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Mozdziak, PE and Giamario, C and Dibner, JJ and McCoy, DW}, year={2004}, month={Jan}, pages={89–94} }
 @article{mozdziak_borwornpinyo_mccoy_petitte_2003, title={Development of transgenic chickens expressing bacterial beta-galactosidase}, volume={226}, ISSN={["1097-0177"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0037370171&partnerID=MN8TOARS}, DOI={10.1002/dvdy.10234}, abstractNote={Abstract}, number={3}, journal={DEVELOPMENTAL DYNAMICS}, author={Mozdziak, PE and Borwornpinyo, S and McCoy, DW and Petitte, JN}, year={2003}, month={Mar}, pages={439–445} }
 @article{mozdziak_dibner_mccoy_2003, title={Glyceraldehyde-3-phosphate dehydrogenase expression varies with age and nutrition status}, volume={19}, ISSN={["0899-9007"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0037403786&partnerID=MN8TOARS}, DOI={10.1016/S0899-9007(02)01006-7}, abstractNote={Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the glycolytic pathway, and it is a popular internal standard for northern blot analysis. We examined GAPDH expression early in life when feed is either provided or not provided to animals.Male broiler chickens were provided a standard starter diet plus Oasis nutritional supplement (fed group; Novus International, St. Louis, MO, USA) or no feed (starved group) for the first 3 d posthatch. Subsequently, the standard starter diet was provided to all chickens between 3 and 7 d posthatch. RNA was extracted from the pectoralis thoracicus, and GAPDH expression was evaluated with quantitative northern analysis.GAPDH expression was significantly (P < 0.05) higher in the fed than in the starved group at 3 d posthatch, suggesting that nutritional manipulations can alter GAPDH transcription. Similarly, GAPDH mRNA levels were significantly (P < 0.05) higher at 7 d posthatch compared with all younger animals, suggesting that GAPDH is developmentally upregulated with advancing age.GAPDH expression changes with age and nutrition status in the early posthatch chick, suggesting that GAPDH is not a proper internal standard for muscle studies using quantitative northern analysis.}, number={5}, journal={NUTRITION}, author={Mozdziak, PE and Dibner, JJ and McCoy, DW}, year={2003}, month={May}, pages={438–440} }
 @article{mozdziak_evans_mccoy_2002, title={Early posthatch starvation induces myonuclear apoptosis in chickens}, volume={132}, ISSN={["1541-6100"]}, DOI={10.1093/jn/132.5.901}, abstractNote={The effect of early posthatch starvation on myonuclear apoptosis was examined in chickens. Male broiler chickens were or were not provided feed for the first 3-d posthatch. Subsequently, all chickens were provided feed for an additional 4-d posthatch. Chickens were killed at 3- and 7-d posthatch, and the pectoralis thoracicus was harvested, fixed and embedded in paraffin. Muscle sections were labeled with the terminal deoxynucleotidyl transferase histochemical staining technique to identify apoptotic nuclei. At 3- and 7-d posthatch, there was a significantly (P < 0.05) smaller myofiber cross-sectional area for the starved compared with the fed chickens. A larger proportion (P < 0.05) of apoptotic nuclei relative to total nuclei was observed in the starved compared to the fed chickens killed at 3-d posthatch, but the proportion of apoptotic nuclei relative to total nuclei did not differ (P > 0.05) between the starved and fed chickens killed at 7-d posthatch. It appears that apoptosis is a mechanism contributing to the smaller myofiber size observed when feed is not provided early posthatch.}, number={5}, journal={JOURNAL OF NUTRITION}, author={Mozdziak, PE and Evans, JJ and McCoy, DW}, year={2002}, month={May}, pages={901–903} }
 @article{mozdziak_walsh_mccoy_2002, title={The effect of early posthatch nutrition on satellite cell mitotic activity}, volume={81}, ISSN={["0032-5791"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0036836103&partnerID=MN8TOARS}, DOI={10.1093/ps/81.11.1703}, abstractNote={Myofiber growth is dependent upon the contribution of new nuclei from the mitotically active satellite cell population. The objective of this study was to examine satellite cell mitotic activity in conjunction with different nutritional paradigms during the early posthatch period. Turkey poults were provided a standard turkey starter diet; the starter diet top-dressed with a hydrated low-fat, highly digestible protein and carbohydrate nutritional hatchling supplement, Oasis; the starter diet top-dressed with Solka-floc dyed green; or no food for the first 3 d posthatch. All birds were fed a standard starter diet during the experimental period. 5-Bromo-2'-deoxyuridine (BrdU) was continuously infused into all treatments (n = 5 all groups) between hatch and 3 d of age. A second group of identically treated poults housed in separate pens (n = 3 to 5) was continuously infused with BrdU between 2 and 9 d of age. Mitotically active satellite cells were identified in the pectoralis thoracicus and quantitated using BrdU immunohistochemistry in combination with computer-based image analysis. Satellite cell mitotic activity was significantly higher (P < or = 0.05) in the birds fed a standard starter diet compared to all other treatments at 3 d posthatch. However, there were no (P > or = 0.05) differences in satellite cell mitotic activity among treatments at 9 d posthatch. The results of the current study suggest that any improvements in meat yield through early nutritional supplementation do not appear to occur through a satellite cell pathway and that there is no compensatory response in the satellite cell population following refeeding after early posthatch starvation.}, number={11}, journal={POULTRY SCIENCE}, author={Mozdziak, PE and Walsh, TJ and McCoy, DW}, year={2002}, month={Nov}, pages={1703–1708} }
 @article{mozdziak_dibner_mccoy_2002, title={The effect of early posthatch starvation on calpain mRNA levels}, volume={133}, ISSN={["1096-4959"]}, url={http://www.scopus.com/inward/record.url?eid=2-s2.0-0036790057&partnerID=MN8TOARS}, DOI={10.1016/S1096-4959(02)00131-8}, abstractNote={The calpain system is a family of calcium activated proteases that degrade myofibrillar protein. Male broiler chickens (Ross) were provided a standard starter diet top-dressed with Oasis((R)) nutritional supplement (fed; Novus International, St. Louis, MO, USA), or they were not provided any feed (starved) for the first 3 days posthatch. Subsequently, the standard starter diet was provided to all chickens between 3 and 7 days posthatch. RNA was extracted from the Pectoralis thoracicus, and skeletal muscle-specific n-calpain-1 (p94) calpain, mu-calpain, and m-calpain expression was evaluated using quantitative Northern analysis. Early posthatch starvation did not (P>0.05) affect calpain mRNA levels on each day examined. Similarly, there were no (P>0.05) changes in mu-calpain or m-calpain mRNA levels between 0 and 7 days posthatch in fed birds. However, p94 calpain mRNA levels were significantly (P<0.05) lower at 7 days posthatch compared to 0 or 2 days posthatch. Therefore, in the early posthatch chicken, it appears that the calpain system may not be affected by the presence of oral nutrition, and that there is an age-related downregulation of p94 calpain mRNA expression.}, number={2}, journal={COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY}, author={Mozdziak, PE and Dibner, JJ and McCoy, DW}, year={2002}, month={Oct}, pages={221–226} }