Michael Jude Stewart

MeSH headings : Animals; Base Sequence; DNA / genetics; DNA-Binding Proteins / genetics; Fibroblasts / immunology; Fibroblasts / virology; Gene Expression Regulation; Humans; Interferon Regulatory Factor-7; Interferon-beta / genetics; Mammalian orthoreovirus 3 / immunology; Mammalian orthoreovirus 3 / pathogenicity; Mice; Mice, Knockout; Myocarditis / genetics; Myocarditis / immunology; Myocarditis / virology; Myocytes, Cardiac / immunology; Myocytes, Cardiac / virology; Orthoreovirus, Mammalian / immunology; Orthoreovirus, Mammalian / pathogenicity; Receptors, Interferon / deficiency; Receptors, Interferon / genetics; Reoviridae Infections / genetics; Reoviridae Infections / immunology; Reoviridae Infections / virology; Reverse Transcriptase Polymerase Chain Reaction

TL;DR: It is suggested that the higher basal IFN-β and ISG expression in myocytes may play an important protective role in cardiac myocytes, and reovirus replicates to higher titers in cardiac fibroblasts than in cardiacMyocytes, suggesting that theIFN- β response controls reov virus replication equivalently in the two cell types. (via Semantic Scholar)

author keywords: myocarditis; reovirus; interferon; PKR

MeSH headings : Animals; Cells, Cultured; Disease Models, Animal; Enzyme Activation; Humans; Interferon-beta / biosynthesis; Mice; Mice, Inbred C57BL; Myocarditis / immunology; Myocarditis / virology; Myocardium / cytology; Orthoreovirus / pathogenicity; Reoviridae Infections / immunology; Reoviridae Infections / virology; Transfection; eIF-2 Kinase / metabolism

TL;DR: KR is demonstrated to be critical for viral induction of IFN-beta in primary cardiac myocyte cultures and loss of PKR leads to an increase in virulence for both myocarditic and nonmyocarditic reoviruses. (via Semantic Scholar)

author keywords: reovirus; double-stranded RNA (dsRNA); viral myocarditis; cardiac myocytes; interferon (IFN); interferon regulatory factor (IRF)

MeSH headings : Animals; Animals, Newborn; Cells, Cultured; DNA-Binding Proteins / biosynthesis; DNA-Binding Proteins / deficiency; DNA-Binding Proteins / genetics; Female; Heart / virology; Interferon Regulatory Factor-1; Interferon-beta / biosynthesis; Interferon-beta / genetics; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Myocardium / metabolism; Myocardium / pathology; Phosphoproteins / biosynthesis; Phosphoproteins / deficiency; Phosphoproteins / genetics; Reoviridae / pathogenicity; Viral Proteins / biosynthesis; Viral Proteins / genetics

TL;DR: Surprisingly, it is found that reovirus failed to induce IRF-1 in the absence of the IFN-alpha/beta response, providing the first evidence that viruses may not induce IRf-1 directly. (via Semantic Scholar)