Glucose‐to‐fructose conversion at high temperatures with xylose (glucose) isomerases from Streptomyces murinus and two hyperthermophilic Thermotoga species
Bandlish, R. K., Hess, J. M., Epting, K. L., Vieille, C., & Kelly, R. M. (2002, August 19). Biotechnology and Bioengineering, Vol. 80, pp. 185–194.
author keywords: Thermotoga; xylose isomerase; Streptomyces murinus; hyperthermophile
MeSH headings : Aldose-Ketose Isomerases / chemistry; Aldose-Ketose Isomerases / classification; Aldose-Ketose Isomerases / metabolism; Bioreactors; Enzyme Activation; Enzymes, Immobilized / metabolism; Fructose / biosynthesis; Glucose / metabolism; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / classification; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / enzymology; Gram-Negative Anaerobic Straight, Curved, and Helical Rods / metabolism; Hot Temperature; Hydrogen-Ion Concentration; Sensitivity and Specificity; Species Specificity; Streptomyces / classification; Streptomyces / enzymology; Thermotoga maritima / classification; Thermotoga maritima / enzymology
topics (OpenAlex): Biofuel production and bioconversion; Diet, Metabolism, and Disease; Pancreatic function and diabetes
TL;DR:
In the presence of both cations, these enzymes showed the potential to catalyze glucose-to-fructose conversion at 80 degrees C with estimated lifetime productivities on the order of 2000 kg fructose per kilogram enzyme, a value competitive with enzymes currently used at 55 degrees to 65 degrees C, but with the additional advantage of higher fructose concentrations.
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