Works (5)
Updated: July 5th, 2023 15:59
2006 journal article
Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens
CELL AND TISSUE RESEARCH, 324(1), 41–53.
Contributors: P. Mozdziak n , Q. Wu n, J. Bradford n, S. Pardue n, n, C. Giamario n, J. Petitte n
author keywords: beta-galactosidase; neurotactin; inverse polymerase chain reaction; genotyping; lacZ; NCSU-Blue1; chicken
MeSH headings : Animals; Animals, Genetically Modified; Base Sequence; Blotting, Southern; Chemokine CX3CL1; Chemokines, CX3C / metabolism; Chick Embryo; Chickens; Female; Genotype; Lac Operon; Male; Membrane Proteins / metabolism; Molecular Sequence Data; Mutagenesis, Insertional; Sequence Homology, Nucleic Acid; beta-Galactosidase / metabolism
TL;DR:
With the identification of homozygous birds, the line of chickens is now designated NCSU-Blue1 and the viral insertion carrying the lacZ gene has been located on chromosome 11 within the predicted gene for neurotactin/fractalkine (CX3CL1).
(via Semantic Scholar)
UN Sustainable Development Goal Categories
2. Zero Hunger
(OpenAlex)
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2005 journal article
Culture of chicken embryos in surrogate eggshells
POULTRY SCIENCE, 84(9), 1477–1482.
author keywords: surrogate eggshell; avian embryo culture; transgenic bird; hatchability
MeSH headings : Animals; Chick Embryo / growth & development; Egg Shell; Time Factors; Tissue Culture Techniques / methods; Tissue Culture Techniques / veterinary; Turkeys
TL;DR:
Hatchability of cultured embryos cultured in turkey eggshells and sealed with Handi Wrap exhibited higher hatchability than cultures sealed with Saran Wrap, which suggested that Handi wrap was an excellent sealant for chick embryos cultured after 3 d of incubation.
(via Semantic Scholar)
academic.oup.com (publisher PDF)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
6. Clean Water and Sanitation
(OpenAlex)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2005 journal article
Enzymatic degradation of a prion-like protein, Sup35NM-His6
ENZYME AND MICROBIAL TECHNOLOGY, 36(5-6), 758–765.
author keywords: yeast prion; Sup35p; Sup35NM; proteases; bovine spongiform encephalopathy
TL;DR:
Results indicated that Sup35NM-His6, being simple and inexpensive to produce and non-pathogenic, can be a potential ideal candidate of prion surrogate protein in the study of PrP Sc prion inactivation and prevention of prions diseases.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Source: Web Of Science
Added: August 6, 2018
2003 journal article
Development of transgenic chickens expressing bacterial beta-galactosidase
DEVELOPMENTAL DYNAMICS, 226(3), 439–445.
Contributors: P. Mozdziak n , n, D. McCoy n & J. Petitte n
author keywords: lacZ; myoblast; muscle; embryo; E. coli
MeSH headings : Animals; Animals, Genetically Modified; Chick Embryo; Chickens; Female; Gene Expression; Gene Transfer Techniques; Lac Operon; Male; Retroviridae / genetics; beta-Galactosidase / genetics
TL;DR:
The replication‐defective retroviral SNTZ vector carrying the lacZ gene with a nuclear localized signal was injected into the subgerminal cavity of freshly laid eggs, and the chickens were screened for the LacZ gene by using the polymerase chain reaction.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
2003 journal article
Transgenic chickens expressing beta-galactosidase hydrolyze lactose in the intestine
JOURNAL OF NUTRITION, 133(10), 3076–3079.
author keywords: lacZ; gene insertion; whey; milk; poultry
MeSH headings : Animal Nutritional Physiological Phenomena; Animals; Animals, Genetically Modified; Chickens / genetics; Galactose / metabolism; Gene Expression; Glucose / metabolism; Hydrolysis; Intestinal Mucosa / enzymology; Intestines / enzymology; Lactase / metabolism; Lactose / metabolism; beta-Galactosidase / genetics; beta-Galactosidase / metabolism
TL;DR:
Overall, it appears that inserting the lacZ gene, which encodes beta-galactosidase, has resulted in a chicken that can utilize lactose as an energy source.
(via Semantic Scholar)
UN Sustainable Development Goal Categories
3. Good Health and Well-being
(Web of Science)
Sources: Web Of Science, ORCID, NC State University Libraries
Added: August 6, 2018
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