@article{taliercio_allen_essenberg_klueva_nguyen_patil_payton_millena_phillips_pierce_et al._2006, title={Analysis of ESTs from multiple Gossypium hirsutum tissues and identification of SSRs}, volume={49}, ISSN={["1480-3321"]}, DOI={10.1139/G05-115}, abstractNote={ In an effort to expand the Gossypium hirsutum L. (cotton) expressed sequence tag (EST) database, ESTs representing a variety of tissues and treatments were sequenced. Assembly of these sequences with ESTs already in the EST database (dbEST, GenBank) identified 9675 cotton sequences not present in GenBank. Statistical analysis of a subset of these ESTs identified genes likely differentially expressed in stems, cotyledons, and drought-stressed tissues. Annotation of the differentially expressed cDNAs tentatively identified genes involved in lignin metabolism, starch biosynthesis and stress response, consistent with pathways likely to be active in the tissues under investigation. Simple sequence repeats (SSRs) were identified among these ESTs, and an inexpensive method was developed to screen genomic DNA for the presence of these SSRs. At least 69 SSRs potentially useful in mapping were identified. Selected amplified SSRs were isolated and sequenced. The sequences corresponded to the EST containing the SSRs, confirming that these SSRs will potentially map the gene represented by the EST. The ESTs containing SSRs were annotated to help identify the genes that may be mapped using these markers.Key words: drought stress, gene annotation, gene mapping, tentative consensus sequence (TC), Xanthomonas campestris. }, number={4}, journal={GENOME}, author={Taliercio, Earl and Allen, Randy D. and Essenberg, Margaret and Klueva, Natalya and Nguyen, Henry and Patil, Mohini A. and Payton, Paxton and Millena, Ana Cecilia M. and Phillips, Angela L. and Pierce, Margaret L. and et al.}, year={2006}, month={Apr}, pages={306–319} } @article{gehring_baynes_wang_craigmill_riviere_2004, title={A web-based decision support system to estimate extended withdrawal intervals}, volume={44}, ISSN={["1872-7107"]}, DOI={10.1016/j.compag.2004.05.002}, abstractNote={All drugs approved for use in food-producing animals have a withdrawal interval to prevent residues in food of animal origin that are potentially harmful to consumers. These withdrawal times must be appropriately extended if the drug is used in an extralabel manner. This paper describes a web-based application that was developed to facilitate the calculation of extended withdrawal intervals based on information in the databases maintained by members of the Food Animal Residue Avoidance Databank (FARAD) and using the Extrapolated Withdrawal Interval Estimator (EWE) algorithm. The implementation of this application was illustrated using a group of antimicrobials that are used in cattle and swine. The use of this application has been limited to staff veterinarians working for FARAD since limitations in the available pharmacokinetic data require that results are interpreted by personnel with in-depth knowledge of the pharmacokinetics of drugs in food-producing animals.}, number={2}, journal={COMPUTERS AND ELECTRONICS IN AGRICULTURE}, author={Gehring, R and Baynes, RE and Wang, J and Craigmill, AL and Riviere, JE}, year={2004}, month={Aug}, pages={145–151} } @article{wang_gehring_baynes_webb_whitford_payne_fitzgerald_craigmill_riviere_2003, title={Evaluation of the advisory services provided by the Food Animal Residue Avoidance Databank}, volume={223}, ISSN={["0003-1488"]}, DOI={10.2460/javma.2003.223.1596}, abstractNote={JAVMA, Vol 223, No. 11, December 1, 2003 A part of its mission to help ensure that foods of animal origin are free of violative chemical residues, the Food Animal Residue Avoidance Databank (FARAD) offers 2 advisory services to veterinary practitioners. The first is a comprehensive online database (VetGRAM) of drugs approved by the US FDA/Center for Veterinary Medicine (CVM) for the treatment of food-producing animals. Second, FARAD offers expert-mediated advice on residue avoidance and mitigation for chemical contamination incidents and the extralabel use of drugs. This service is provided by FARAD pharmacologists and toxicologists, who can be reached by e-mail as well as a toll-free telephone number.}, number={11}, journal={JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION}, author={Wang, JM and Gehring, R and Baynes, RE and Webb, AI and Whitford, C and Payne, MA and Fitzgerald, K and Craigmill, AL and Riviere, JE}, year={2003}, month={Dec}, pages={1596–1598} }